Journal of Eexercise & Organ Cross Talk

Crosstalk between antioxidant enzymes and interleukin-10 in rats fed a high-fat diet: The impact of exercise and lctobacillus bifidus supplementation

Document Type : Original Article

Authors

Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract
The present study will investigate the changes and correlation between the antioxidant enzyme superoxide dismutase (SOD) and the expression of interleukin 10 (IL-10) in rats fed a high-fat diet (HFD) after 6 weeks of aerobic exercise supplemented with the probiotic Lactobacillus bifidus. 40 male Wistar rats were divided into 5 groups (8 each): healthy control, high-fat diet (HFD), HFD+aerobic exercise (Tr), HFD+probiotic supplementation (Sup), and HFD+Tr+Sup. The rats in the HFD group received a special high-fat diet for 2 months prior to the main protocol. The rats in the exercise groups ran on a rodent treadmill for 6 weeks, 5 days a week. Lactobacillus bifidus supplementation was gavaged at a dose of 1 cc to the rats in the supplementation groups after each exercise session. Finally, the rats were sacrificed, and the IL-10 variable was measured using the Real-Time PCR method. The results of the present study indicated that the high-fat diet increased cholesterol and triglyceride levels (p<0.05). The HFD and HFD+Sup groups showed a significant decrease in SOD compared to the healthy control group (p<0.05). All treatment groups demonstrated a significant increase in SOD compared to the HFD group (p<0.05). The HFD+Tr and HFD+Tr+Sup groups showed a significant increase in IL-10 compared to the HFD group (p<0.05). However, the correlation between SOD and IL-10 was not confirmed (p>0.05). There was no correlation between the antioxidant enzyme and IL-10. Nevertheless, exercise and Lactobacillus bifidus were able to regulate the lipid profile, SOD, and IL-10 after a high-fat diet.

What is already known on this subject?

Bifidobacterium, Lactobacillus, and Enterococcus faecalis are probiotics that have beneficial effects in the maintenance treatment of human intestinal diseases.

 

What this study adds?

There was no correlation between the antioxidant enzyme and IL-10. Nevertheless, exercise and Lactobacillus bifidus were able to regulate the lipid profile, SOD, and IL-10 after a high-fat diet.

Keywords

Subjects


Acknowledgements

None.

Funding

None of the researchers received financial support.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval This study was conducted under the supervision of the Animal Ethics Committee of the Islamic Azad University, Science and Research Branch, Tehran, Iran, with ethical code IR.IAU.SRB.REC.1402.187.

Informed consent Not applicable

Author contributions

Conceptualization:S.R.R,M.GH,H.A,F.GH.; Methodology: S.R.R, M.GH, Software: H.A, F.GH.; Validation: M.GH.; Formal analysis: H.A, F.GH.; Investigation: M.GH.; Resources: S.R.R.; Data curation: S.R.R, M.GH, H.A, F.GH.; Writing - original draft: S.R.R.; Writing - review & editing: S.R.R, M.GH, H.A, F.GH.; Visualization: S.R.R, M.GH, H.A, F.GH.; Supervision: M.GH.; Project administration: M.GH.; Funding acquisition: M.GH.

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Volume 4, Issue 1
Winter 2024
Pages 58-66

  • Receive Date 02 January 2024
  • Revise Date 21 February 2024
  • Accept Date 21 March 2024