Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

Long-term adaptation in lipolysis due to aerobic interval training in rats with metabolic syndrome

Document Type : Original Article

Authors

Mohammad Reza Yousefi 1
Golpasand Kohzadi 2
Mahnaz Omidi 3

1 Assistant Professor, Sports Science Department, Ilam Branch, Islamic Azad University, Ilam, Iran.

2 2- Graduated from the Department of Sports Sciences, Ilam Branch, Islamic Azad University, Ilam, Iran.

3 Assistant Professor, Department of Sports Sciences, Ilam Branch, Islamic Azad University, Ilam, Iran.

https://doi.org/10.22122/jeoct.2024.464527.1113
Abstract
Exercise training is known to enhance lipolysis in response to hormonal challenges, but the impact of different exercise modalities on fat metabolism remains unclear. This study aimed to investigate the effects of eight weeks of interval training on enzymes and hormones involved fat breakdown in rats with metabolic syndrome, focusing on the expression of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL), as well as plasma levels of glucagon and epinephrine. Thirty-five male rats were randomly divided into five groups (7 rats/group): Experimental Group1 (6 weeks of fructose solution), Experimental Group2 (6 weeks of fructose+8 weeks of interval training), Experimental Group3 (14 weeks of fructose), Control Group1 (6 weeks without intervention), and Control Group2 (14 weeks without intervention). Western blot analysis assessed HSL and ATGL expression, while ELISA measured plasma glucagon and epinephrine levels. Fructose consumption for 6 and 14 weeks induced metabolic syndrome in male rats, leading to a significant reduction in HSL and ATGL protein expression (P = 0.024 and P = 0.034, respectively). Interval training for 8 weeks significantly increased HSL and ATGL levels (P = 0.011 and P = 0.025, respectively), indicating enhanced fat tissue breakdown. Moreover, interval training significantly decreased glucagon levels (P = 0.015), though it did not affect epinephrine levels (P = 0.159). Interval training effectively reverses some metabolic syndrome-associated impairments in fat metabolism, specifically increasing key lipolytic enzymes and reducing glucagon levels. This suggests a potential therapeutic role for interval training in managing metabolic syndrome.

What is already known on this subject?

What is already known on this subject?

Interval training effectively reverses some metabolic syndrome-associated impairments in fat metabolism, specifically increasing key lipolytic enzymes and reducing glucagon levels. This suggests a potential therapeutic role for interval training in managing metabolic syndrome.

 

What this study adds?

It seems that high-intensity interval training can reduces markers of metabolic syndrome, including insulin resistance and triglyceride levels.

Keywords

Lipolysis
ATGL
HSL
Epinephrine
Glucagon
Metabolic syndrome

Subjects

Acknowledgements

This article is taken from the Master's thesis in exercise physiology from Islamic Azad University, ilam Branch. We are grateful to all those who cooperated with us in this research.

Funding

None of the researchers received financial support.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval This research was approved by the animal care and use committee at University of Medical Sciences of Ilam, Iran (Approval reference number: IR.MEDILAM.AEC.1401.008  ).

Informed consent Animal study

Author contributions

Conceptualization: M.R.Y.; Methodology: M.R.Y, M.O. Software: M.O., M.R.Y.; Validation: M.R.Y, M.O. Formal analysis: G.K., M.O.; Investigation: F.R., M.E.Z, G.O.; Resources: M.O.Y, G.K. Data curation: M.O., G.K.; Writing - original draft: G.K., M.R.Y.; Writing - review & editing: M.R.Y, M.O.; Visualization: M.O.Y, G.K.; Supervision: M.R.Y.; Project administration: G.K., M.O.; Funding acquisition: M.R.Y.

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Journal of Exercise & Organ Cross Talk
Volume 4, Issue 1
Winter 2024
Pages 49-57

  • Receive Date 07 January 2024
  • Revise Date 01 March 2024
  • Accept Date 15 March 2024

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