Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

Investigating the correlation between glutathione peroxidase and Interleukin-15 following aerobic exercise and probiotic supplementation in obese rats fed a high-fat diet

Document Type : Original Article

Authors

Mahsa Pekand
Mandana Gholami
Hossein Abednatanzi
Farshad Ghazalian

Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

https://doi.org/10.22122/jeoct.2024.476113.1121
Abstract
We investigate the effect of 6 weeks of aerobic training and probiotic supplementation on glycemic index and the correlation between glutathione peroxidase (GPX) and interleukin 15 (IL-15) in rats fed a high-fat diet. 40 male Wistar rats were divided into 5 groups (8 each): healthy control, high-fat diet (HFD), HFD+ aerobic exercise (Tr), HFD+ probiotic supplement (Sup) and HFD+Tr+Sup. Rats in the HFD group received a special high-fat diet for 2 months before the main protocol. The rats in the training groups ran on a rodent treadmill for 6 weeks, 5 days a week. Lactobacillus bifidus supplement was gavage to the rats of the supplemented groups in the amount of 109 CFU/ml after each round of training. The results of the statistical test showed that the HFD groups had a significant decrease in GPX compared to the healthy control group (p<0.05). However, only the HFD+Tr+Sup group showed a significant increase in GPX compared to the HFD and HFD+Tr groups (p<0.05). In contrast, HFD+Sup showed a significant increase in IL-15 gene expression compared to the healthy control group. Compared to the HFD group, the HFD+Tr and HFD+Tr+Sup groups showed a decrease and the HFD+Sup group showed a significant increase in IL-15 gene expression (p<0.05). The correlation between GPX and IL-15 was not confirmed (p<0.05). It seems that exercise training and lactobacillus supplementation are effective in regulating glucose, GPX and IL-15 indices after HFD. However, more studies should be done for the relationship and cross talk between antioxidant indices and IL-15.

What is already known on this subject?

Probiotics are currently used for the treatment or prevention of human diseases, conditions, and syndromes. They have also been shown to have a positive impact on neuroinflammation and pain, as well as on infections from seasonal diseases.

 

What this study adds?

Exercise training, may regulate both circulating IL-15 and GPx levels separately. Probiotics that enhance GPx activity in the liver may also influence IL-15 levels, either directly or indirectly, through their effects on oxidative stress and inflammation.

Keywords

Correlation
Exercise training
Probiotics
High-fat diet
Interleukin-15

Subjects

Acknowledgements

None.

Funding

None of the researchers received financial support.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval This study was conducted under the supervision of the Animal Ethics Committee of the Islamic Azad University, Science and Research Branch, Tehran, Iran, with ethical code IR.IAU.SRB.REC.1402.188.

Informed consent Not applicable

Author contributions

Conceptualization: M.P, M.GH, H.A, F.GH.; Methodology: M.P, M.GH,.; Software: H.A, F.GH.; Validation: M.GH.; Formal analysis: H.A, F.GH.; Investigation: M.GH.; Resources: M.P.; Data curation: M.P, M.GH, H.A, F.GH.; Writing - original draft: M.P.; Writing - review & editing: M.P, M.GH, H.A, F.GH.; Visualization: M.P, M.GH, H.A, F.GH.; Supervision: M.GH.; Project administration: M.GH.; Funding acquisition: M.GH.

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Journal of Exercise & Organ Cross Talk
Volume 4, Issue 1
Winter 2024
Pages 40-48

  • Receive Date 11 January 2024
  • Revise Date 06 March 2024
  • Accept Date 12 March 2024

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