Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

Effects of resistance, endurance, and concurrent exercise on carnitine palmitoyltransferases 1, 2 and fatigue index in obese diabetic rats

Document Type : Original Article

Authors

Afsaneh Mohammadi 1
Sedigheh Hosseinpour Delavar 1
Ali Zabet 1
Mehdi Bagzadeh 2
Forogh Mohammadi 3

1 Department of Exercise Physiology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.

2 Department of Exercise Physiology, Eslamabad- E- Gharb branch, Islamic Azad University, Eslamabad -E-Gharb, Iran.

3 Department of Veterinary, Agriculture Faculty, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.

https://doi.org/10.22122/jeoct.2024.469370.1117
Abstract
The objective of this study was to evaluate the effects of endurance, resistance, and Concurrent endurance and resistance training on fat oxidation and fatigue levels in obese diabetic rats. A total of forty overweight rats (average weight of 380 ± 50 g) were randomly assigned to five distinct groups. Diabetes was induced through peritoneal administration of Streptozotocin at a dosage of 55 mg/kg body weight. Endurance training was conducted via moderate-intensity treadmill running (50%-70% of maximum running capacity) for a duration of eight weeks, with sessions lasting one hour per day, five days a week. Resistance training involved ladder climbing at 50-70% of maximum load, also for eight weeks, with five sessions per week, comprising 15 climbs per session and a one-minute rest interval between climbs. The Concurrent training regimen alternated between treadmill and ladder exercises. Levels of CPT1 and CPT2 were quantified using rat CPT1 and CPT2 ELISA kits, both prior to and 48 hours following the training period. Fatigue indices were assessed as well. All three training modalities significantly influenced CPT1 levels (F=93.35, P=0.001) and led to a notable increase in CPT2 levels (F=26.76, P=0.001). Additionally, significant alterations in fatigue indices were observed (F=26.12, P=0.001). The findings suggest that aerobic, resistance, and Concurrent training regimens can enhance metabolic function and prolong the duration until fatigue in elderly diabetic rats. Nonetheless, the extent of these improvements is somewhat contingent upon the specific type of exercise performed, with aerobic and Concurrent training demonstrating a more pronounced effect compared to resistance training.

What is already known on this subject?

It is not uncommon for individuals to develop certain conditions like diabetes as they grow older. Moreover, it is widely recognized that diabetes has an impact on lipid metabolism. Previous studies on lifestyle have indicated that engaging in physical activities could potentially help maintain overall health and slow down the natural aging process, ultimately preventing premature aging.

 

What this study adds?

This investigation demonstrated significant correlations between fatigue and CPT1 and CPT2. Therefore, participating in endurance, resistance, or a combination of exercises may be an effective strategy to prevent age-related muscle loss and enhance metabolism, leading to improved fatigue levels. Furthermore, the study established connections between CPT1, CPT2 levels, athletic performance, and fatigue.

Keywords

CPT1
CPT2
Training
Diabetes
Body Weight

Subjects

Acknowledgements

The writers would like to extend their gratitude to all those who cooperated with us in writing this article.

Funding

None of the researchers received financial support.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval This research was approved by the Medical Ethics Committee of Kermanshah Branch of Islamic Azad University with the code IR.IAU.HSH.REC.1402.142. Ethical principles were completely observed with respect to feeding the rats, providing living conditions for them, taking care of them, and disposing of their carcasses.

Informed consent Not applicable

Author contributions

Conceptualization: A.M, S.H.D, A.Z, M.B, F.M.; Methodology: A.M, S.H.D.; Software: A.Z, M.B, F.M.; Validation: A.M.; Formal analysis: A.M, S.H.D.; Investigation: A.Z.; Resources: A.M, S.H.D, A.Z, M.B, F.M.; Data curation: A.M, S.H.D, A.Z, M.B, F.M.; Writing - original draft: A.M, S.H.D.; Writing - review & editing: A.Z, M.B, F.M.; Visualization: A.M, S.H.D.; Supervision: A.Z.; Project administration: A.Z.; Funding acquisition: A.Z.

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Journal of Exercise & Organ Cross Talk
Volume 4, Issue 1
Winter 2024
Pages 31-39

  • Receive Date 23 January 2024
  • Revise Date 05 March 2024
  • Accept Date 20 March 2024

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