Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

The relationship between wnt/β-catenin pathway and muscle-enriched myokine musclin following combined exercise and curcumin consumption in rats with glioblastoma multiforme

Document Type : Original Article

Authors

Semaneh Afsharyousefi 1
Yaser Kazemzadeh 2
Sanaz Mirzayan Shanjani 2

1 PhD student, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.

2 Exercise Physiology Department, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.

https://doi.org/10.22122/jeoct.2024.468197.1114
Abstract
This study aims to investigate the effect of resistance-aerobic exercise and curcumin consumption on brain wnt/β-catenin expression and their correlation with muscle-enriched myokine musclin in rats with glioblastoma multiforme. Fourty male Wistar rats were used in this study. After getting familiarized with the research environment, the rats were randomly divided into five groups of eight: healthy control, tumor control, tumor + resistance-aerobic exercise, tumor + Nano-curcumin supplement, and tumor + aerobic-resistance exercise + Nano-curcumin supplement. For tumor induction, glioblastoma stereotaxic injection was utilized in the frontal cortex. The exercise training group did aerobic training and resistance training 3 days a week for 4 weeks. Nano curcumin supplement was gavage with a dose of 80 mg/kg for 4 weeks, 5 days a week. The brain histology changes of the groups with GBM injection confirmed brain tumor induction compared to the healthy control group (p<0.05). The expression of wnt/β-catenin genes in all tumor groups showed a significant increase compared to the healthy control group. Treatment with resistance-aerobic exercise and curcumin (especially the combination of both) revealed the greatest decrease in the expression of wnt/β-catenin genes compared to the tumor group. Wnt mRNA had a negative correlation with musclin mRNA in Tumor+N. cur group (r=-0.905, p=0.001). It seems that the use of nano supplements along with combined resistance-aerobic exercise can control the wnt/β-catenin signaling pathway in brain tumor tissue. In addition, the cross talk between muscle and brain should be more studied with considering different myokine.

What is already known on this subject?

Brain cancer is a devastating and very debilitating form of cancer. In 2014, there were an estimated 23,000 new cases of brain and nervous system cancer in the United States. However, over time this number increased.

 

What this study adds?

use of nano supplements along with combined resistance-aerobic exercise can control the wnt/β-catenin signaling pathway in brain tumor tissue. In addition, the cross talk between muscle and brain should be more studied with considering different myokine.

Keywords

Brain cancer
Musclin
Resistance-aerobic exercise
Curcumin
Gene expression

Subjects

Acknowledgements

None

Funding

None.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval All stages of this study were under the supervision of the Ethics Committee of Islamic Azad University, Tehran, Iran (ethics code: IR.IAU.VARAMIN.REC.1402.018).

Informed consent Animal study.

Author contributions

Conceptualization: S.A.; Methodology: Y.K., SMS.; Software: S.A., Y.K.; Validation: S.M.S.; Formal analysis: Y.K.; Investigation: S.A.; Resources: S.M.S.; Data curation: Y.K.; Writing - original draft: S.A.; Writing - review & editing: S.M.S.; Visualization: S.A.; Supervision: Y.K.; Project administration: S.M.S.; Funding acquisition: Y.K.

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Journal of Exercise & Organ Cross Talk
Volume 4, Issue 1
Winter 2024
Pages 12-21

  • Receive Date 03 January 2024
  • Revise Date 06 March 2024
  • Accept Date 17 March 2024

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