Correlation between the muscle, blood and heart level of Irisin in exercise-trained rats with Nano selenium supplementation: A rat model of COPD

Document Type : Original Article


1 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran.

2 Anatomy Department of Medical Faculty, Baqiyatallah University of Medical Science, Tehran, Iran.

3 Tobacco Control Research Center (TCRC), Iranian Anti-Tobacco Association, Tehran, Iran.


The aim of this study was to considering the correlation between the muscle fibronectin type III domain-containing protein 5 (FNDC5), blood and heart level of Irisin in exercise-trained rats with Nano selenium supplementation after intraperitoneal injection of cigarette smoke extract induced chronic obstructive pulmonary disease (COPD). To this end, 49 male Wistar rats (8 weeks old) were divided into seven groups: control, SeNPs (2.5 mg/kg b.w by oral gavage, 3 days/week, 6 weeks), AIT (49 min/day, 5 days/week for 6 weeks, interval), SeNPs+AIT, CSE (150 µL by IP injection, 1 day/week for 6 weeks), CSE+AIT, and CSE+SeNPs+AIT. The results of the present study showed that CSE injection caused inflammation and damage to lung tissue, especially alveoli, compared to the healthy group. In other words, based on the histological examination of cigarette smoke extract, it was able to cause lung tissue damage similar to COPD, and doing exercise and taking nanoselenium antioxidant supplement could control these lung tissue damage. Pearson's correlation method was used to investigate the relationship between muscle FNDC5, serum and heart Irisin, and the results of this correlation were not significant in different groups (p>0.05). It seems that exercising and taking nanoselenium supplements can increase Irisin levels in serum and heart tissue by expanding muscle contraction and increasing muscle FNDC5. However, the relationship of this factor in muscle and heart crosstalk should be investigated more closely.

What is already known on this subject?

Irisin, which is mostly secreted from muscle tissue, regulates mitochondrial energy, glucose metabolism, fatty acid oxidation, and fat browning.


What this study adds?

Exercise training and Nano selenium supplementation are able to increase the serum and heart levels of Irisin.


Main Subjects


We would like to express our gratitude to the Tobacco Control Research Center (TCRC) of the Iranian Anti-Tobacco Association for supporting this research project.



Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval Experiments were performed according to NIH Guidelines for the animal study on Wistar rats weighing about 180–220 g bred at the animal laboratory, at Baqiyatallah University of medical sciences, Tehran, Iran (ethical code: IR.BMSU.REC.1400.117).

Informed consent Animal study.

Author contributions

Conceptualization: Z.F.; Methodology: M.R.M., J.R.S.; Software: Z.F., Sh. M.; Validation: Sh.M.; Formal analysis: Z.F.; Investigation: Sh.M.; Resources: M.R.M.; Data curation: J.R.S.; Writing - original draft: Z.F.; Writing - review & editing: Sh.M.; Visualization: M.R.M.; Supervision: Sh.M.; Project administration: Z.F.; Funding acquisition: M.R.M.

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