Over the past few decades, research involving brain-gut crosstalk has rapidly evolved to become an important topic in preclinical and clinical research. In the health and sports environment, a paradigm shift in the bidirectional interactions between the gut–brain is essential for exercise-induced rehabilitation strategies and preventive interventions. However, there are caveats to consider when interpreting the mechanism by which physical exercise can exert its effects on the brain–gut axis, given that different exercise protocols (e.g., duration and intensity) have variable effects on this crosstalk. Therefore, an integrative perspective of the exercise-induced gut–brain communication pathway will be discussed in this chapter.