Muscle and serum antioxidant cross talk following curcumin and light resistance training during strenuous endurance training in male Wistar rats

Document Type : Original Article


1 Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.

2 Ph.D Student in Sport Physiology, University of Kurdistan, Sanandaj, Iran.


It has been proven that strenuous endurance training increases oxidative stress in body. This study investigated the effects of curcumin supplementation and light resistance training during 8 weeks of endurance training on muscle and serum antioxidant capacity and lipid peroxidation of male wistar rats. 44 male Wistar rats (weight: 254.31±17.72 g and age: 8 weeks) were randomly divided to 6 groups; Control (n=6, sham), Curcumin (n=6), Endurance (n=8), Endurance-curcumin (n=8), Endurance-Resistance (n=8), and Endurance-curcumin-resistance (n=8). Endurance training performed on rodent treadmill for 8 weeks and 5 sessions a week. The speed and duration of running were 10 m/min and 30 min at first week. The intensity and duration reached to the 35 m/min and 70 min up to the last week. Resistance training (8 weeks, 2 sessions / week) performed on vertical ladder (with 30-70% BW). The animals received curcumin supplement by sub peritoneal injection (8 weeks, 3 sessions / week, 30 mg/kg.Bw). Superoxide dismutase (SOD) enzyme activity was measured by Elisa kit and Malondialdehyde (MDA) was measured by the thiobarbituric acid reactive substances (TBARS).  The results of this study showed that strenuous endurance training (p<0.05) reduces the serum levels of SOD significantly, and caused a significant increase in the lipid peroxidation (MDA in muscle and serum). Curcumin supplementation and light resistance training could increase antioxidant enzymes activity (SOD) and decrease the MDA levels. The prolonged strenuous endurance training can induce oxidative stress and curcumin supplementation along with light resistance training could restore antioxidant enzymes activity and decrease the MDA levels.

What is already known on this subject?

Endurance training induces oxidative stress.


What this study adds?

Curcumin supplementation and light resistance training prevent oxidative stress.


Main Subjects

Afzalpour, M., Gharakhanlou, R., Gaeini, A., Mohebi, H., & Hedayati, S. (2006). The effects of vigorous and moderate aerobic exercise on the serum arylesterase activity and total antioxidant capacity in non-active healthy men. Research on Sport Science. URL:
Akpinar, D., Yargicoglu, P., Derin, N., Aliciguzel, Y., & Agar, A. (2008). The effect of lipoic acid on antioxidant status and lipid peroxidation in rats exposed to chronic restraint stress. Physiological Research, 57(6), 893-901. doi:
Balcı, S. S., Pepe, H., Güney, S., Özer, Ç., & Revan, S. (2012). Effects of gender, endurance training and acute exhaustive exercise on oxidative stress in the heart and skeletal muscle of the rat. Chinese Journal of Physiology, 55(4), 236-244. doi:
Banaeifar, A. A., Gorzi, A., Hedayati, M., Nabiollahi, Z., Rahmani Moghaddam, N., & Khantan, M. (2011). Effect of an 8-week resistance training program on acetylcholinesterase activity in rat muscle. KAUMS Journal ( FEYZ ), 15(4), 316-321. URL:
Chang, Y. C., & Chuang, L. M. (2010). The role of oxidative stress in the pathogenesis of type 2 diabetes: From molecular mechanism to clinical implication. American Journal of Translational Research, 2(3), 316. URL:
Dabidi, R. V., Hosseinzadeh, S., Mahjoub, S., Hosseinzadeh, M., & Myers, J. (2013). Endurance exercise training and diferuloyl methane supplement: Changes in neurotrophic factor and oxidative stress induced by lead in rat brain. Biology of Sport, 30(1), 41-46. doi:
Gomes, E. C., Silva, A. N., & Oliveira, M. R. d. (2012). Oxidants, antioxidants, and the beneficial roles of exercise-induced production of reactive species. Oxidative Medicine and Cellular Longevity, 2012. doi:
 Goodarzi, B., & KHosravi, A. (2013). The Effects of simultaneous 8 weeks Astragalus sp/Euphorbia Cheriradenia Honey supplementation and endurance training on membrane lipid peroxidation of erythrocytes after a bout acute exhaustive treadmill exercise in rats. European Academic Research, 1(2), 114-137. URL:
Gorzi, A., & Asadi, M. (2020). Useful effects of curcumin supplementation on gastric superoxide dismutase activity and serum malondialdehyde level during endurance training in male wistar rats. Zahedan Journal of Research in Medical Sciences, 22(2). doi:
Gorzi, A., Asadi, M., Voltarelli, F., & Shamsi, M. M. (2021). Effects of curcumin on antioxidant capacity and gastric mucosal injury following strenuous endurance training in rats. Comparative Exercise Physiology, 17(1), 17-24. doi:
Gorzi, A., & Ekradi, S. (2020). The effect of intake duration of curcumin supplementation during strenuous endurance training on GPX activity and MDA levels of liver, heart and skeletal muscle in male Wistar rats. Sport Physiology, 12(46), 139-156. doi:
Gorzi, A., Ekradi, S., & Rahmani, A. (2018). The effect of high intensity endurance training on antioxidant defense and lipid peroxidation of male Wistar rats. Journal of Sport Biosciences, 10(3), 333-345. doi:
Gorzi, A., Tofighi, A., & Amiri, B. (2018). The effects of curcumin supplementation on oxidative stress induced during strenuous endurance training on the kidney and lung tissues. Scientific Journal of Kurdistan University of Medical Sciences, 23(5), 1-11.  URL:
Güzel, N. A., Hazar, S., & Erbas, D. (2007). Effects of different resistance exercise protocols on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. Journal of Sports Science & Medicine, 6(4), 417-422. URL:
Halle, M., Berg, A., Baumstark, M., & Keul, J. (1999). Association of physical fitness with LDL and HDL subfractions in young healthy men. International Journal of Sports Medicine, 20(07), 464-469. doi:
Joo, Y. I., Sone, T., Fukunaga, M., Lim, S. G., & Onodera, S. (2003). Effects of endurance exercise on three-dimensional trabecular bone microarchitecture in young growing rats. Bone, 33(4), 485-493. doi:
Kaya, H., Sezik, M., Ozkaya, O., Dittrich, R., Siebzehnrubl, E., & Wildt, L. (2004). Lipid peroxidation at various estradiol concentrations in human circulation during ovarian stimulation with exogenous gonadotropins. Hormone and Metabolic Research, 36(10), 693-695. doi:
Kerksick, C., & Willoughby, D. (2005). The antioxidant role of glutathione and N-acetyl-cysteine supplements and exercise-induced oxidative stress. J Int Soc Sports Nutr, 2(2), 38-44. doi:
Kettawan, A., Wongsansri, K., Chompoopong, S., & Rungruang, T. (2012). Antioxidant and antiplasmodial activities of curcuma longa and aegle marmelos on malaria infeced mice (in vitro and in vivo). URL:
Lamina, S., Ezema, C. I., Theresa, A. I., & Anthonia, E. U. (2013). Effects of free radicals and antioxidants on exercise performance. Oxidants and Antioxidants in Medical Science, 2(2), 83-91. doi:
Lee, C. (2000). Antioxidant ability of caffeine and its metabolites based on the study of oxygen radical absorbing capacity and inhibition of LDL peroxidation. Clinica Chimica Acta, 295(1), 141-154. doi:
Liu, Z. C., Yang, Z.X., Zhou, J. S., Zhang, H. T., Huang, Q. K., Dang, L. L., . . . Tao, K. S. (2014). Curcumin regulates hepatoma cell proliferation and apoptosis through the Notch signaling pathway. International Journal of Clinical and Experimental Medicine, 7(3), 714-718. URL:
Miller, N. J., Rice-Evans, C., Davies, M. J., Gopinathan, V., & Milner, A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84, 407-407. doi:
Morillas-Ruiz, J., Zafrilla, P., Almar, M., Cuevas, M., Lopez, F., Abellan, P., . . . Gonzalez-Gallego, J. (2005). The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. European Journal of Applied Physiology, 95(5-6), 543-549. doi:
Powers, S. K., & Jackson, M. J. (2008). Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production. Physiological Reviews, 88(4), 1243-1276. doi:
Samy Ali Hussein Aziza, S. A. A. A. a. H. A. (2014). Chemopreventive effect of curcumin on oxidative stress, antioxidant status, DNA fragmentation and caspase-9 gene expression in 1,2-dimethylhydrazine-induced colon cancer in rats. American Journal of Biochemistry and Molecular Biology, 4, 22-34. doi:
Sen, C. K. (1995). Oxidants and antioxidants in exercise. Journal of Applied Physiology, 79(3), 675-686. doi:
Silva, L., Bom, K., Tromm, C., Rosa, G., Mariano, I., Pozzi, B., . . . Pinho, R. (2013). Effect of eccentric training on mitochondrial function and oxidative stress in the skeletal muscle of rats. Brazilian Journal of Medical and Biological Research, 46(1), 14-20. doi:
Sonwa, M. M., & König, W. A. (2001). Chemical study of the essential oil of Cyperus rotundus. Phytochemistry, 58(5), 799-810. doi:
Teixeira, V., Valente, H., Casal, S., Marques, F., & Moreira, P. (2009). Antioxidant status, oxidative stress, and damage in elite trained kayakers and canoeists and sedentary controls. Int J Sport Nutr Exerc Metab, 19(5), 443-456. doi:
Thirumalai, T., Therasa, S. V., Elumalai, E., & David, E. (2011). Intense and exhaustive exercise induce oxidative stress in skeletal muscle. Asian Pacific Journal of Tropical Disease, 1(1), 63-66. doi:
Tofigi, A., Gorzi, A., & Amin, B. (2017). The effect of curcumin supplementation accompanied by light resistance training during 8 weeks of endurance training on SOD and MDA levels of kidney tissues in male Wistar rats. Journal of Applied Exercise Physiology, 13(25), 75-86. doi:
Volume 1, Issue 2
September 2021
Pages 86-92
  • Receive Date: 20 July 2021
  • Revise Date: 06 September 2021
  • Accept Date: 20 September 2021
  • First Publish Date: 20 September 2021