This study’s major purpose was to examine the impact of two various exercise intensities on glucagon-like peptide-1 and endoplasmic reticulum stress in teenagers with type 2 diabetes. Twenty teenagers with T2DM were recruited in the trial and randomly assigned to one of two groups: HIT (n = 10) or LIT (n = 10). At a heart rate of less than 45 percent, the low-intensity intense exercise group participated in aerobic activity training. Interval training with a heart rate reserve of at least 80% was used by the high-intensity intermittent training group. Both groups had the same weekly energy expenditure (1,200 kcal/week). The nositol required enzyme 1 and X-box binding protein-1 improved in both groups, but the difference in improvement between the two groups was not statistically significant. High-intensity interval training led to a significant decrease in body fat percentage among participants. After 12 weeks, the C-peptide levels had risen and varied considerably between the two groups. According to the homeostasis model assessment, there were significant differences between the groups in fasting glucose and insulin resistance, and leptin levels reduced after 12 weeks. The 12-week rise in glucagon-like peptide-1 was different across the groups. In teenagers with type 2 diabetes mellitus, high-intensity intermittent exercise training may enhance glycemic management, physical body composition, glucagon-like peptide-1, and endoplasmic reticulum stress.