Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

The effect of aerobic interval-style continuous training combined with CoQ10 supplementation on MDA and TGF-β levels in inactive obese men

Articles in Press

Document Type : Original Article

Authors

peyman Akraminia 1
Abdolali Banaiefar 2
Vahid Imanipour 3
Hadi Khoran 4

1 Tehtan

2 Associated Professor, Department of exercise physiology, ST.C., Islamic Azad university, Tehran, Iran.

3 Department of Physical Education, ST.C., Islamic Azad University, Tehran, Iran

4 Department of Physical Education, ZARA.C., Islamic Azad University, Markazi, Iran

10.22122/jeoct.2026.577937.1197
Abstract
Background and Aim: Obesity is associated with chronic low-grade inflammation and elevated oxidative stress. This study investigated the independent and combined effects of an 8-week aerobic interval-style continuous training program and CoQ10 supplementation on serum TGF-β and MDA concentrations in inactive obese men.

Methods: Sixty inactive obese men (aged 30–45 years) were randomly assigned to four groups (n=15 each): control (CONT), CoQ10 supplementation (SUP; 100 mg/day), training (TR), and combined training + supplementation (TR+SUP). The supervised training protocol was performed three sessions per week for 8 weeks with progressive intensity. Fasting venous blood samples were collected before and after the intervention. Serum MDA was measured using colorimetric assay, and TGF-β was quantified via ELISA. Data was analyzed using two-way repeated-measures ANOVA.

Results: Significant time × exercise interactions were observed for both MDA (F(1,56) = 28.793, p < 0.001) and TGF-β (F(1,56) = 30.617, p < 0.001), with reductions in the exercise groups (MDA: exercise groups from 210 nmol/L to 156 nmol/L; TGF-β: from 35 nmol/L to 30 pg/mL). Time × supplementation interactions were also significant but smaller (MDA: η_p² = 0.082; TGF-β: η_p² = 0.068). No significant three-way interaction was detected for either marker.

Conclusion: These findings highlight aerobic interval-style continuous training as a cornerstone intervention for mitigating obesity-related oxidative stress and fibrotic signaling. CoQ10 supplementation produced a small independent reduction in both markers, but no synergistic or additive interaction with exercise was observed.

Keywords

Training
coenzyme Q10
malondialdehyde
transforming growth factor-beta
Subjects
Journal of Exercise & Organ Cross Talk

Articles in Press, Accepted Manuscript
Available Online from 01 September 2026

  • Receive Date 24 February 2026
  • Revise Date 12 May 2026
  • Accept Date 16 June 2026

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