Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

The effect of moderate-intensity intermittent training and coenzyme Q10 supplementation on serum VEGF, total antioxidant capacity, and hydrogen peroxide in sedentary obese men

Articles in Press

Document Type : Original Article

Authors

peyman Akraminia 1
Abdolali Banaiefar 2
Vahid Imanipour 3
Hadi Khoran 4

1 Tehtan

2 Associated Professor, Department of exercise physiology, ST.C., Islamic Azad university, Tehran, Iran.

3 Department of Physical Education, ST.C., Islamic Azad University, Tehran, Iran

4 Department of Physical Education, ZARA.C., Islamic Azad University, Markazi, Iran

10.22122/jeoct.2026.577930.1196
Abstract
Background: Sedentary obesity is associated with chronic inflammation, oxidative stress, and impaired angiogenesis, leading to endothelial dysfunction. Interval training and CoQ10 each improve redox balance and vascular health, possibly via inter-organ crosstalk, but their combined effects in sedentary obese men are unclear.

Methods: Sixty sedentary obese men (30–45 years) were randomized into four groups (n=15): control, CoQ10 (100 mg/day), moderate-intensity intermittent training (3×/week, 8 weeks), and training + CoQ10. Serum VEGF, TAC, and H₂O₂ were measured pre/post-intervention via ELISA and colorimetric assays. Data were analyzed with repeated-measures ANOVA (α=0.05). Effect sizes (partial η²) were reported.

Results: Training significantly raised VEGF (p < 0.001, η² = 0.363) and TAC (p < 0.001, η² = 0.290) , while strongly reducing H₂O₂ (p < 0.001, η² = 0.520) . CoQ10 showed no significant main or interactive effects on TAC or H₂O₂; however, a significant three-way interaction (time × exercise × supplement) was observed for VEGF (p = 0.001, η² = 0.187). Post-hoc analysis revealed that the exercise-only group showed a greater increase in VEGF compared to the combined group, though the direct comparison between TR and TR+SUP at post-intervention did not reach statistical significance (p > 0.05).

Conclusion: Eight weeks of interval training robustly improves VEGF and redox homeostasis in sedentary obese men, possibly through myokines/exerkines enhancing muscle–adipose–endothelial crosstalk and HIF-1α/VEGF signaling. CoQ10 provides no added benefit for most outcomes and may be associated with a modest attenuation of the exercise-induced VEGF response.

Keywords

training
Coenzyme Q10
Vascular endothelial growth factor
Total antioxidant capacity
Hydrogen peroxide
Subjects
Journal of Exercise & Organ Cross Talk

Articles in Press, Accepted Manuscript
Available Online from 01 September 2026

  • Receive Date 24 February 2026
  • Revise Date 12 May 2026
  • Accept Date 16 June 2026

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