Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

The effect of exercise training with Nano selenium supplementation on LDHA, LDHB genes and LDHA/LDHB ratio at breast tumor tissue of mouse model

Articles in Press

Document Type : Original Article

Authors

Mohadeseh Akaberi 1
Mandana Gholami 2
Hossein Shirvani 3
Farshad Ghazalian 1
Hossein Abednatanzi 1

1 Department of Physical Education and Sport Sciences, SR.C., Islamic Azad university, Tehran, Iran.

2 Associate Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Exercise Physiology Research Center, life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

10.22122/jeoct.2026.579301.1200
Abstract
This study investigated the effects of high intensity interval training (HIIT), nano-selenium supplementation, and their combination on the expression of LDHA, LDHB, and the LDHA/LDHB ratio in mice breast tumor tissue. Female mice (n = 32) were inoculated with mammary adenocarcinoma cells and randomly assigned to four groups (n = 8 each): tumor control (Tu), tumor + HIIT (Tu + Ex), tumor + nano-selenium (Tu + Nsel, 0.5 mg/kg/day orally), and tumor + HIIT + nano-selenium (Tu + Ex + Nsel). HIIT was performed on a treadmill (30 min/day, 5 days/week) for four weeks. Real time PCR was used to measure LDHA and LDHB mRNA expression in breast tumor tissue. The LDHA/LDHB ratio was calculated, and data were analyzed using one way ANOVA followed by Tukey’s post hoc test. One way ANOVA revealed significant differences among groups for LDHA expression (F = 38.66, p < 0.0001). Compared to the Tu group, all intervention groups (Tu + Ex, Tu + Nsel, and Tu + Ex + Nsel) showed a significant increase in LDHA expression (p < 0.05). The greatest increase was observed in the combined treatment group (Tu + Ex + Nsel), which was significantly higher than both Tu + Ex and Tu + Nsel (p < 0.001). For the LDHA/LDHB ratio, a significant overall effect was found (F = 163.87, p < 0.0001).

Keywords

Breast cancer
LDHA
LDHB
Nano-selenium
Exercise
Subjects
Journal of Exercise & Organ Cross Talk

Articles in Press, Accepted Manuscript
Available Online from 01 June 2026

  • Receive Date 22 March 2026
  • Revise Date 10 May 2026
  • Accept Date 12 May 2026

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