Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

The combined effects of resistance training and pineapple extract on intratumoral NF-κB, LIN28B, and systemic TNF-α in a murine melanoma model

Document Type : Original Article

Authors

Adeleh Afshar
Hossein Abednatanzi
Farshad Ghazalian
Mandana Gholami

Department of physical education and sport sciences, SR.C., Islamic Azad university, Tehran, Iran.

https://doi.org/10.22122/jeoct.2025.548128.1170
Abstract
This study investigated the effects of resistance training and pineapple extract consumption on intratumoral NF-κB and LIN28B gene expression and serum TNF-α levels in a murine C57 melanoma model. Twenty C57BL/6 mice were allocated into four groups (n=5/group): melanoma tumor control (MT), MT with resistance training (MT+RT), MT with pineapple extract (MT+PJ), and MT with combined intervention (MT+RT+PJ). The RT protocol and PJ administration (300 mg/kg/day via gavage) were conducted for six weeks’ post-tumor induction. Serum TNF-α was quantified by ELISA, and tumor gene expression of NF-κB and LIN28B was analyzed via RT-PCR. Data were analyzed using one-way ANOVA followed by Tukey's post hoc test. All three intervention groups exhibited a significant downregulation of NF-κB and LIN28B gene expression in tumor tissue compared to the MT control group (p<0.05). Conversely, serum TNF-α levels were significantly elevated in the intervention groups relative to the control (p<0.05). Resistance training and pineapple extract consumption, both individually and in combination, significantly modulated pro-tumorigenic pathways by suppressing intratumoral NF-κB and LIN28B expression, despite an observed increase in systemic TNF-α.

What is already known on this subject?

The tumor microenvironment, marked by chronic inflammation, plays a critical role in melanoma development and progression through complex interactions between tumor cells and inflammatory mediators.

 

What this study adds?

Resistance training and pineapple extract consumption individually and combined attenuate melanoma progression by downregulating intratumoral NF-κB and LIN28B expression while elevating systemic TNF-α to promote anti-tumor immunity.

Keywords

Resistance training
Pineapple extract
Melanoma
NF-&kappa
B
LIN28B
TNF-&alpha
Cytokines
Mouse model

Subjects

Acknowledgements

None

Funding

None

Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Compliance with ethical standards

Conflict of interest The authors declare that there is no conflict of interest in the present research.

Ethical approval This study received approval under the research ethics code IR.SSRC.REC.1401.340 from the Azad University of Sciences and Research.

Informed consent Animal study.

Author contributions

Conceptualization: A.A., H.A, F.Gh., M.GH., Methodology: A.A., H.A, F.Gh., M.GH., Software: A.A., H.A, F.Gh., M.GH., Validation: A.A., H.A, F.Gh., M.GH.,; Formal analysis: A.A., H.A, F.Gh., M.GH.,; Investigation: A.A., H.A, F.Gh., M.GH.,; Resources: A.A., H.A, F.Gh., M.GH.,; Data curation: A.A., H.A, F.Gh., M.GH.,; Writing - original draft: A.A., H.A, F.Gh., M.GH.,; Writing–review & editing: A.A., H.A, F.Gh., M.GH.,; Visualization: A.A., H.A, F.Gh., M.GH.,; Supervision: H.A.; Project administration: H.A.,.; Funding acquisition: H.A.

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Journal of Exercise & Organ Cross Talk
Volume 5, Issue 3
Summer 2025
Pages 116-122

  • Receive Date 19 June 2025
  • Revise Date 03 September 2025
  • Accept Date 05 September 2025

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