Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

Impact of high-intensity interval training on ASC inflammasome, lipid profile and their correlation in diabetic rat model

Document Type : Original Article

Authors

Reza Sheikh 1
Mohammad Reza Tabandeh 2, 3
Saeid Shakerian 1

1 Department of sport physiology, Faculty of sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Department of Basic Sciences, Division of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

3 Stem Cells and Transgenic Technology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

https://doi.org/10.22122/jeoct.2025.503653.1142
Abstract
In Diabetes (Di) exercise training especially high-intensity interval training (HIIT) reduce blood lipids and improve anti-inflammatory status. In this study, we aimed to investigate the impact of high-intensity interval training on ASC inflammasome, lipid profile, and their correlation in diabetic rat models. Twenty rats were divided into four groups, including a control group, HIIT, Di, and Di+HIIT (n=5 in each group). Diabetes was induced using a combination of a high-fat diet (HFD) and STZ. Wistar rats in the exercise groups were subjected to high-intensity interval training for eight weeks. After sample collection, liver tissue was removed and weighed. Serum levels of lipid profile were measured by special kit. Protein expression of the apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) in liver tissue was determined by Western blot. The results of the present study showed that diabetes induction significantly increased LDL, cholesterol, triglyceride and ASC levels and decreased HDL levels (p<0.05), whereas HIIT exercise training with diabetes somewhat adjusted the lipid profile and decreased the ASC inflammasome levels (p<0.05). In the correlation analysis, only the correlation between ASC and triglycerides was confirmed in the Di+HIIT group (p=0.043, r=0.997). Diabetes induction adversely affects lipid profiles and increases ASC inflammasome levels. However, high-intensity interval training (HIIT) appears to mitigate these effects by improving the lipid profile and reducing ASC levels. Notably, a significant positive correlation between ASC and triglycerides was observed in the diabetic rats undergoing HIIT, suggesting a link between inflammation and lipid metabolism.

What is already known on this subject?

The role of inflammation and inflammasomes in the pathophysiology and progression of diabetes mellitus (DM), which includes both type 1 (T1D) and type 2 (T2D), is crucial.

 

What this study adds?

the implementation of HIIT in diabetic subjects appears to positively influence these lipid parameters and reduce ASC inflammasome levels.  

Keywords

Inflammasome
ASC
Correlation
HIIT
Liver
Diabetes

Subjects

Acknowledgements

None.

Funding

No sources of funding were sought or awarded for this study.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval The ethical code for animal care (EE.1401.2.24.173155) from Ahvaz University approved all phases of the experiment.

Informed consent Animal study.

Author contributions

Conceptualization: R.Sh., S.Sh., M.R.T.; Methodology: R.Sh., S.Sh., M.R.T.; Software: R.Sh.; Validation: S.Sh., Formal analysis; Investigation: M.R.T.; Resources: R.Sh., S.Sh., M.R.T.; Data curation: M.R.T.; Writing - original draft: R.Sh.; Writing – review & editing: R.Sh., S.Sh., M.R.T.; Visualization: R.Sh., S.Sh., M.R.T.; Supervision: S.Sh. Project administration: R.Sh.; Funding acquisition: S.Sh.

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Journal of Exercise & Organ Cross Talk
Volume 4, Issue 3
Summer 2024
Pages 177-183

  • Receive Date 31 July 2024
  • Revise Date 11 September 2024
  • Accept Date 13 September 2024

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