Journal of Exercise & Organ Cross Talk
Journal of Eexercise & Organ Cross Talk

Impact of water resistance training and dark chocolate on IGF-1 and FOXO3 gene expressions in elderly women: Correlation analysis of variables

Document Type : Original Article

Authors

Maryam Mousavi 1
Farshad Ghazalian 2
Shahram Soheili 3
Mandana Gholami 2
Hossein Abed Natanzi 2

1 Ph.D Student, Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Department of Physical Education and Sport Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.

https://doi.org/10.22122/jeoct.2024.486946.1131
Abstract
Resistance exercises and nutritional strategy are the best ways to prevent with age- related muscle atrophy (sarcopenia) by improving the physiological function of skeletal muscle. This study investigated the effect of 8 weeks of Water resistance training with consumption of dark chocolate on the changes and correlation of IGF-1 and FOXO3 in elderly women. In this study, 40 sedentary elderly women with an age range of 60-73 years were randomly divided into four groups: 1. water resistance training, 2. water resistance training + dark chocolate, 3. dark chocolate, 4. control. The subjects of the training groups did water resistance training for eight weeks. During these 8 weeks, the dark chocolate groups consumed 30 grams of 83% dark chocolate every day, and the control group received neither exercise nor Dark chocolate. Blood sampling was done 48 hours before the study and after the end of the last training session. Expressions of IGF-1 and FOXO3 were measured by real time-PCR method. After 8 weeks of water resistance training and consumption of dark chocolate, the expression of insulin-like growth factor 1 (IGF-1) gene increased significantly compared to the pretest (p<0.05). However, FOXO3 did not change significantly (p>0.05). These findings underscore the potential of targeted exercise and dietary interventions in enhancing muscle health among the elderly, although further research is needed to understand the implications of unchanged FOXO3 in this context.

What is already known on this subject?

Resistance exercises and nutritional strategy are the best ways to prevent with age- related muscle atrophy (sarcopenia) by improving the physiological function of skeletal muscle.

 

What this study adds?

increased IGF-1 may lead to a downregulation of FOXO3 activity, thereby potentially mitigating the effects of sarcopenia—a condition characterized by muscle loss and functional decline in older adults.

Keywords

Water resistance training
Dark chocolate
Elderly
IGF-1
FOXO3

Subjects

Acknowledgements

None.

Funding

The funding for this research was provided by the author.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval The proposal was registered with the approval of the Research Vice Presidency and the Specialized Ethics Committee in Biomedical Research at Islamic Azad University, Science and Research Branch, Tehran, with the ethics code 1403.088REC.SRB.IAU.IR (IRCT: 20240519061840N1).

Informed consent Not applicable

Author contributions

Conceptualization: M.M., H.A., M.Gh.; Methodology: Sh.S., H.A., F.Gh.; Software: H.A.; Validation: M.M., Formal analysis; Investigation: M.Gh., F.Gh.; Resources: Sh.S., H.A.; Data curation: M.M., H.A., M.Gh.; Writing - original draft: Sh.S.; Writing – review & editing: H.A.; Visualization: M.Gh.; Supervision: H.A. Project administration: H.A.; Funding acquisition: M.M.

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Journal of Exercise & Organ Cross Talk
Volume 4, Issue 2
Spring 2024
Pages 117-125

  • Receive Date 03 March 2024
  • Revise Date 20 June 2024
  • Accept Date 22 June 2024

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