High-intensity interval training increases thermogenesis and metabolism through changes in regulatory proteins

Document Type : Original Article


1 Department of Biological Sciences in Sport and Health, Faculty of Sport Sciences and Health, Tehran university, Tehran, Iran

2 Department of Biological Sciences in Sport and Health, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran


The present study aimed to investigate the effect of 4 weeks of high-intensity interval training on the amounts of metabolic proteins like Sarcolipine, PGC1α and FNDC5 in SOL and EDL muscles of male Wistar rats. In the present study, 14 adult male Wistar rats were randomly divided into experimental (n = 7) and control (n = 7) groups. The training group performed high-intensity interval training for 4 weeks and 5 sessions per week, including high-intensity (90% vVO2max) and low-intensity (45% vVO2max) two-minute intervals. Finally, the SOL and EDL muscles of the research groups were extracted, and also independent statistical t-test (p <0.05) was used for statistical analysis. The results showed that the amounts of SLN, PGC1α, and FNDC5 proteins in the experimental group were significantly different from the control group in SOL muscle, but the amounts of FNDC5 protein in the EDL muscle of the HIIT group were not significantly different from the control group. The results showed that by observing the changes in the amounts of regulatory proteins related to cellular metabolism due to high-intensity interval training, this training method could be suggested to increase metabolism and improve the lipid oxidation process in a short time.

What is already known on this subject?

Physical exercises with high intensity can increase the metabolism in the resting situation, which increases the energy consumption in the form of heat generation through the increase of the regulatory proteins in the muscles.


What this study adds?

HIIT method increases calcium pump regulatory proteins, which subsequently consume more energy in the sarcoplasmic reticulum to regulate the calcium cycle by increasing the sarcolipin protein, which increases mitochondrial biogenesis and ultimately produces and consumes more ATP.


Main Subjects


This research is based on the Master's thesis of the Faculty of Sports and Health Sciences, Shahid Beheshti University, Tehran, in collaboration with the laboratory of the Neuroscience Center of Shahid Beheshti University of Medical Sciences. The authors hereby express their gratitude to Shahid Beheshti University and the personnel of the Center for Neuroscience and Sports Sciences of Shahid Beheshti University.



Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval This study has been approved by the Ethics Committee of the Research Institute of Physical Education and Sports Sciences with code IR.SBU.REC.1401.039.

Informed consent Animal study.

Author contributions

Conceptualization: M.F., A.J., M.N; Methodology: M.F., M.T.; Software: A.J.; Validation: M.F., M.T.; Formal analysis: M.N., M.F.; Investigation: M.T.; Resources: M.F.; Data curation: M.F., M.T.; Writing - original draft: M.N., M.F.; Writing - review & editing: M.F., M.N.; Visualization: A.J.; Supervision: M.N.; Project administration: A.J., M.N.; Funding acquisition: M.N.

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