The effect of herbal supplement and exercise training on plasma lipid profile in diabetic male rats

Document Type : Original Article

Authors

1 PhD student in sports physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Ph.D. in Biochemistry and Sports Metabolism, Department of Physical Education, Faculty of Humanities, Islamic Azad University, Ahvaz Branch, Iran.

Abstract

The present study was conducted to investigate the effect of six weeks of intermittent exercise and curcumin consumption on the lipid profile of diabetic male rats. In an experimental trial, 30 male Wistar rats were randomly divided into six groups (5 rats in each group): control, diabetic, healthy intermittent exercise, intermittent exercise +diabetes, curcumin +diabetes and curcumin+ exercise +diabetes. Diabetes was induced by intraperitoneal injection of streptozotocin poison (50 mg/kg). Intermittent training consisted of 5-12 bouts of intense work (75 to 100% of maximum speed) of 60 seconds with active rest intervals of 75 seconds, six days a week for six weeks. Curcumin was fed to animals by gavage at a dose of 50 mg per kilogram of body weight. After six weeks, unconscious animals and blood samples were collected from their hearts. The data were analyzed using ANOVA statistical test and Bonferroni post hoc test. Induction of diabetes caused an increase in cholesterol, TG and LDL levels (P=0.01). Intermittent training and curcumin, each alone, caused them to decrease (P=0.01). In addition, diabetes decreased HDL while intermittent exercise increased it, but curcumin supplementation did not have a significant effect. The effect of combining interval training with curcumin supplement was significant only for TG and did not have a significant effect on other data. Exercise training and curcumin can have an anti-inflammatory effect with reducing lipid profile and increasing HDL in diabetic animal.

What is already known on this subject?

Diabetes is a metabolic disease characterized by a hyperglycemic state caused by defects in insulin action (insulin resistance), insulin secretion, or both.

 

What this study adds?

both intermittent exercise and curcumin supplementation could improve blood lipid indices, which are disturbed in diabetic conditions, to a certain extent.

Keywords

Main Subjects

Acknowledgements

This article is taken from the Master's thesis under the number (10621423972032) of Ahvaz University of Science and Research, which was conducted at the animal house center of Shahid Chamran University of Ahvaz. We are grateful for the sincere cooperation and support of both universities. In addition, the authors of this article have no conflict of interest with the publication of this article.

Funding

None.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval Experimental protocols were approved by the Ethics Committee of Islamic Azad University, Ahvaz, Iran (Ethcal code: IR.IAU.AHVAZ.REC.1400.056).

Informed consent Animal study.

Author contributions

Conceptualization: M.G., R.SH.; Methodology: M.G., R.SH.; Software: R.SH.; Validation: M.G.; Formal analysis: M.G.; Investigation: M.G.; Resources: M.G.; Data curation: M.G., R.SH.; Writing - original draft: M.G., R.SH.; Writing – review & editing: M.G.; Visualization: M.G.; Supervision: M.G.; Project administration: M.G., R.SH.; Funding acquisition: R.SH.

References

Alguwaihes, A. M., Al-Sofiani, M. E., Megdad, M., Albader, S. S., Alsari, M. H., Alelayan, A., . . . Jammah, A. A. (2020). Diabetes and Covid-19 among hospitalized patients in Saudi Arabia: a single-centre retrospective study. Cardiovascular Diabetology, 19, 1-12. doi: https://doi.org/10.1186/s12933-020-01184-4
Altobelli, E., Angeletti, P. M., Marziliano, C., Mastrodomenico, M., Giuliani, A. R., & Petrocelli, R. (2021). Potential therapeutic effects of curcumin on glycemic and lipid profile in uncomplicated type 2 diabetes—a meta-analysis of randomized controlled trial. Nutrients, 13(2), 404. doi: https://doi.org/10.3390/nu13020404
Artha, I. M. J. R., Bhargah, A., Dharmawan, N. K., Pande, U. W., Triyana, K. A., Mahariski, P. A., . . . Manuaba, I. B. A. P. (2019). High level of individual lipid profile and lipid ratio as a predictive marker of poor glycemic control in type-2 diabetes mellitus. Vascular Health and Risk Management, 149-157. URL: https://www.tandfonline.com/doi/full/10.2147/VHRM.S209830
Association, A. D. (2014). Diagnosis and classification of diabetes mellitus. Diabetes Care, 37(Supplement_1), S81-S90. doi: https://doi.org/10.2337/dc14-S081
Awadalla, H., Noor, S. K., Elmadhoun, W. M., Bushara, S. O., Almobarak, A. O., Sulaiman, A. A., & Ahmed, M. H. (2018). Comparison of serum lipid profile in type 2 diabetes with and without adequate diabetes control in Sudanese population in north of Sudan. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 12(6), 961-964. doi: https://doi.org/10.1016/j.dsx.2018.06.004
Charrin, E., Dubé, J. J., Connes, P., Pialoux, V., Ghosh, S., Faes, C., . . . Martin, C. (2018). Moderate exercise training decreases inflammation in transgenic sickle cell mice. Blood Cells, Molecules, and
and Diseases, 69, 45-52.  doi: https://doi.org/10.1016/j.bcmd.2017.06.002
Fujita, N., Aono, S., Karasaki, K., Sera, F., Kurose, T., Fujino, H., & Urakawa, S. (2018). Changes in lipid metabolism and capillary density of the skeletal muscle following low-intensity exercise training in a rat model of obesity with hyperinsulinemia. PLoS ONE, 13(5), e0196895. doi: https://doi.org/10.1371/journal.pone.0196895
Kumar, S. P., & Sandhya, A. (2018). A study on the glycemic, lipid and blood pressure control among the type 2 diabetes patients of north Kerala, India. Indian Heart Journal, 70(4), 482-485. doi: https://doi.org/10.1016/j.ihj.2017.10.007
Kurita, T., & Makino, Y. (2013). Novel curcumin oral delivery systems. Anticancer Research, 33(7), 2807-2821. URL: https://ar.iiarjournals.org/content/33/7/2807.short
Last, A. R., Ference, J. D., & Menzel, E. R. (2017). Hyperlipidemia: drugs for cardiovascular risk reduction in adults. American Family Physician, 95(2), 78-87.
Little, J. P., Safdar, A., Wilkin, G. P., Tarnopolsky, M. A., & Gibala, M. J. (2010). A practical model of low‐volume high‐intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. The Journal of Physiology, 588(6), 1011-1022. doi: https://doi.org/10.1113/jphysiol.2009.181743
Mantzorou, M., Pavlidou, E., Vasios, G., Tsagalioti, E., & Giaginis, C. (2018). Effects of curcumin consumption on human chronic diseases: A narrative review of the most recent clinical data. Phytotherapy Research, 32(6), 957-975. doi: https://doi.org/10.1002/ptr.6037
Muscella, A., Stefano, E., & Marsigliante, S. (2020). The effects of exercise training on lipid metabolism and coronary heart disease. American Journal of Physiology-Heart and Circulatory Physiology, 319(1), H76-H88. doi: https://doi.org/10.1152/ajpheart.00708.2019
Na, L. X., Li, Y., Pan, H. Z., Zhou, X. L., Sun, D. J., Meng, M., . . . Sun, C. H. (2013). Curcuminoids exert glucose‐lowering effect in type 2  diabetes by decreasing serum free fatty acids: A double‐blind, placebo‐controlled trial. Molecular Nutrition & Food Research, 57(9), 1569-1577. doi: https://doi.org/10.1002/mnfr.201200131
Naghizadeh, H., & Heydari, F. (2023). The effect of 12 weeks of HIIT and curcumin consumption on oxidative indices in obese men with type-2 diabetes mellitus. Journal of Sport and Exercise Physiology, 15(4/67), 81.
Neerati, P., Devde, R., & Gangi, A. K. (2014). Evaluation of the effect of curcumin capsules on glyburide therapy in patients with type‐2 diabetes mellitus. Phytotherapy Research, 28(12), 1796-1800. doi: https://doi.org/10.1002/ptr.5201
Rashidlamir, A., Alizadeh, A., Ebrahimiatri, A., & Dastani, M. (2013). The effect of four-week period of aerobic exercise with cinnamon consumption on lipoprotein indicates and blood sugar in diabetic female patients (type 2). SSU Journals, 20(5), 605-614.
Shamsi-Goushki, A., Mortazavi, Z., Mirshekar, M. A., Mohammadi, M., Moradi-Kor, N., Jafari-Maskouni, S., & Shahraki, M. (2020). Comparative effects of curcumin versus nano-curcumin on insulin resistance, serum levels of apelin and lipid profile in type 2 diabetic rats. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 2337. doi: https://doi.org/10.2147/DMSO.S247351
Szkudelski, T. (2001). The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research, 50(6), 537-546. URL: https://www.biomed.cas.cz/physiolres/2001/issue6/pdf/szkudelski.pdf
Wang, L., Zhang, B., Huang, F., Liu, B., & Xie, Y. (2016). Curcumin inhibits lipolysis via suppression of ER stress in adipose tissue and prevents hepatic insulin resistance [S]. Journal of Lipid Research, 57(7), 1243-1255. doi: https://doi.org/10.1194/jlr.M067397
Wang, N., Liu, Y., Ma, Y., & Wen, D. (2017). High-intensity interval versus moderate-intensity continuous training: Superior metabolic benefits in diet-induced obesity mice. Life Sciences, 191, 122-131. doi: https://doi.org/10.1016/j.lfs.2017.08.023
 
Journal of Exercise & Organ Cross Talk
Volume 3, Issue 2
June 2023
Pages 86-92

Files

History

  • Receive Date: 29 April 2023
  • Revise Date: 12 June 2023
  • Accept Date: 20 June 2023

Share

How to cite

Statistics