The effect of herbal supplement and exercise training on plasma lipid profile in diabetic male rats

Document Type : Original Article


1 PhD student in sports physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Ph.D. in Biochemistry and Sports Metabolism, Department of Physical Education, Faculty of Humanities, Islamic Azad University, Ahvaz Branch, Iran.


The present study was conducted to investigate the effect of six weeks of intermittent exercise and curcumin consumption on the lipid profile of diabetic male rats. In an experimental trial, 30 male Wistar rats were randomly divided into six groups (5 rats in each group): control, diabetic, healthy intermittent exercise, intermittent exercise +diabetes, curcumin +diabetes and curcumin+ exercise +diabetes. Diabetes was induced by intraperitoneal injection of streptozotocin poison (50 mg/kg). Intermittent training consisted of 5-12 bouts of intense work (75 to 100% of maximum speed) of 60 seconds with active rest intervals of 75 seconds, six days a week for six weeks. Curcumin was fed to animals by gavage at a dose of 50 mg per kilogram of body weight. After six weeks, unconscious animals and blood samples were collected from their hearts. The data were analyzed using ANOVA statistical test and Bonferroni post hoc test. Induction of diabetes caused an increase in cholesterol, TG and LDL levels (P=0.01). Intermittent training and curcumin, each alone, caused them to decrease (P=0.01). In addition, diabetes decreased HDL while intermittent exercise increased it, but curcumin supplementation did not have a significant effect. The effect of combining interval training with curcumin supplement was significant only for TG and did not have a significant effect on other data. Exercise training and curcumin can have an anti-inflammatory effect with reducing lipid profile and increasing HDL in diabetic animal.

What is already known on this subject?

Diabetes is a metabolic disease characterized by a hyperglycemic state caused by defects in insulin action (insulin resistance), insulin secretion, or both.


What this study adds?

both intermittent exercise and curcumin supplementation could improve blood lipid indices, which are disturbed in diabetic conditions, to a certain extent.


Main Subjects


This article is taken from the Master's thesis under the number (10621423972032) of Ahvaz University of Science and Research, which was conducted at the animal house center of Shahid Chamran University of Ahvaz. We are grateful for the sincere cooperation and support of both universities. In addition, the authors of this article have no conflict of interest with the publication of this article.



Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval Experimental protocols were approved by the Ethics Committee of Islamic Azad University, Ahvaz, Iran (Ethcal code: IR.IAU.AHVAZ.REC.1400.056).

Informed consent Animal study.

Author contributions

Conceptualization: M.G., R.SH.; Methodology: M.G., R.SH.; Software: R.SH.; Validation: M.G.; Formal analysis: M.G.; Investigation: M.G.; Resources: M.G.; Data curation: M.G., R.SH.; Writing - original draft: M.G., R.SH.; Writing – review & editing: M.G.; Visualization: M.G.; Supervision: M.G.; Project administration: M.G., R.SH.; Funding acquisition: R.SH.

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