Effect of incremental interval training on levels of Fasl protein in lung tissue of mature male Wistar rats: Does exercise training reduce lung inflammation?

Document Type : Original Article


1 Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Mazandaran University, Babolsar, Iran.

2 PhD Student of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.


Intense exercise training is associated with Lung inflammation. Fasl protein on the cell surface is responsible for the initiation of the inflammatory response that finally leads to cell death at the site of inflammation, and can be interpreted as Fasl induced apoptosis. Therefore, the aim of this study was to investigate the effects of increasing and intense interval exercise training on Fasl levels of mature rat lungs. 30 rats within three weeks of birth with mean weight 68±9 g were randomly divided into three basic, control, and exercise groups. Increasing interval training for 6 sessions per week, each session 30 minutes at a speed of 15 to 70 meters per min was employed and Fasl levels were measured using the kitby Elisa method. The data were analyzed with two-way ANOVA and LSD test was done at p≤0.05 significant level. The results showed that Fasl protein levels in the interval training group compared to baseline group increased by 23.75 % and was significant (p≤0/05). However, although the amount of this protein in the interval training group compared to the control group was high, this value was not significant. In addition, Fasl protein levels in the control group compared to the baseline group increased by13.58 % and was significant (p≤0.05). The findings indicated that intense and prolonged exercise training causes damage of the respiratory tract, and in turn, leads to the increased levels of Fasl.

What is already known on this subject?

Using the best training methods to achieve maximum adaptation in the least possible time is one of the goals of coaches and athletes.


What this study adds?

Intense exercise increasing Fasl levels in lung tissue that can induce inflammation.


Main Subjects





Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval Experimental protocols were approved by the Ethics Committee of Mazandaran University, Babolsar, Iran.

Informed consent Animal study.

Author contributions

Conceptualization: S.A., R.SH.; Methodology: S.A., R.SH.; Software: R.SH.; Validation: S.A.; Formal analysis: S.A.; Investigation: .M., Z.H.F.; Resources: .M., Z.H.F.; Data curation: S.A., R.SH.; Writing - original draft: S.A., R.SH.; Writing – review & editing: S.A.; Visualization: S.A., R.SH.; Supervision: S.A.; Project administration: S.A., R.SH.; Funding acquisition: S.A.

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