The effect of high-intensity interval training on IL-22 and STAT3 gene expression of liver tissue in steatosis animal model

Document Type : Original Article


1 M.Sc. Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran.

2 Assistant Professor, Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran.

3 PhD Student of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, Bu-Ali Sina University, Hamedan , Iran.


Inflammation is a major component of almost all acute and chronic liver disorders, including non-alcoholic fatty liver disease. This study aimed to investigate the effect of high-intensity interval training on IL-22 and STAT3 gene expression of liver tissue in steatosis animal model. In this experimental study, 32 rats (weighing 200-250 gr) were selected and randomly divided into 4 groups including healthy control, fatty liver, HIIT and fatty liver + HIIT group. Rats were infected with fatty liver by oral tetracycline at a dose of 140 mg/kg (soluble in 2 ml of water) for 7 days. The HIIT exercise program performed on treadmill five sessions per week for 5 weeks. The IL-22 and STAT3 gene expressions in the liver tissue of samples were measured by Real Time PCR. Data were analyzed by One-way ANOVA and Tukey post hoc tests at significance level P <0.05. The results showed that the gene expression of IL-22 in liver tissue in HIIT group and fatty liver + HIIT was significantly lower than that in the fatty liver group (P = 0.001). Also, the gene expression of STAT3 in liver tissue in HIIT group and fatty liver + HIIT was significantly higher than that in the fatty liver group (P = 0.001). According to the results, the HIIT training program seems to help improve the liver steatosis.

What is already known on this subject?

Intense interval exercise led to a significant decrease in the expression of the IL-22 gene and a significant increase in the expression of the STAT3 gene in the steatosis animal model.



This article is taken from the master's thesis, and by this means, sincere thanks and appreciation are given to all the people who participated in this research.


Main Subjects


This article is taken from the master's thesis, and by this means, sincere thanks and appreciation are given to all the people who participated in this research.


There is no funding to report.

Compliance with ethical standards

Conflict of interest The authors declare that they have no conflict of interest.

Ethical approval All procedures performed in studies involving animal participants were in accordance with the ethical standards of the institutional and/or national research committee and with the Declaration of Helsinki.

Informed consent done.

Author contributions

Conceptualization: V.E.Gh, M.A, T.G.L, H.Ch; Methodology: V.E.Gh, M.A.; Software: H.Ch.; Validation: T.G.L.; Formal analysis: V.E.Gh.; Investigation: H.Ch.; Resources: V.E.Gh.; Data curation: T.G.L.; Writing - original draft: M.A.; Writing - review & editing: H.Ch, T.G.L.; Visualization: M.A.; Supervision: M.A.; Project administration: V.E.Gh.; Funding acquisition: M.A.

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