The effects of high-intensity interval training on the expression of interleukin-10 and STAT3 genes in the intestinal tissue of rats affected by hepatic steatosis

Document Type : Original Article

Authors

1 Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

2 Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran

Abstract

Hepatic steatosis is increasingly being recognized as an important pathological feature of disease that commonly reported in patients with inflammatory bowel disease. This study aimed to investigate the effects of High-Intensity Interval Training (HIIT) on the expression of interleukin-10 and Signal transducer and activator of transcription 3 (STAT3) genes in intestinal tissue in an animal model of fatty liver. In this experimental study, 24 rats (weighing 200-250 gr) were selected and randomly divided into 3 groups including healthy control, fatty liver, and fatty liver + HIIT, groups. In order to induce fatty liver, oral tetracycline 140 mg/kg/day in 2 mL of water in form of a solution was given to the rats by gavage for 7 days. HIIT exercise program performed on treadmill five sessions per week for 5 weeks. Data were analyzed by one-way ANOVA and Tukey post hoc tests. P<0.05 was considered significant. The results showed that IL10 gene expression in HIIT groups was significantly lower than in the fatty liver group (p<0.0001). Also, the expression of the STAT3 gene in intestinal tissue was significantly upper in HIIT groups than that in the fatty liver group (p<0.0001). Regulation of IL-10 and STAT3 gene expression in fatty liver-induced adipose tissue can be modulated by HIIT exercise. Therefore, intense interval training can be considered as a non-pharmacological strategy in the treatment of fatty liver.

What is already known on this subject?

Hepatic steatosis is increasingly being recognized as an important pathological feature of disease that commonly reported in patients with inflammatory bowel disease.

 

What this study adds?

High-intensity Interval Training balanced the expression of anti-inflammatory cytokine genes through cellular mechanism. Therefore, HIIT can probably help in the treatment of fatty liver.

Keywords

Main Subjects

References

Batista Júnior, M. L., Lopes, R. D., Seelaender, M. C. L., & Lopes, A. C. (2009). Anti-inflammatory effect of physical training in heart failure: role of TNF-α & IL-10. Arqu brasileiros cardiologia, 93,692-700. URL: https://www.scielo.br/j/abc/a/ZnCZGjfSbFKWBPF7SDrFKJj/abstract/?lang=en
Bonen, D. K., & Cho, J. H. (2003). The genetics of inflammatory bowel disease. Gastroenterology, 124(2), 521-536. doi: https://doi.org/10.1053/gast.2003.50045
Calbet, L., Fernández-Pérez, L., De Pablos-Velasco, P., Santana, A., Ponce-González, J. A., Morales-Alamo, D., . . . Chapinal, E. (2011). Is sprint exercise a leptin signaling mimetic in human. J Appl Physiol, 111, 715-725. doi: https://doi.org/10.1152/japplphysiol.00805.2010
Dorneles, G. P., Haddad, D. O., Fagundes, V. O., Vargas, B. K., Kloecker, A., Romão, P. R., & Peres, A. (2016). High intensity interval exercise decreases IL-8 and enhances the immunomodulatory cytokine interleukin-10 in lean and overweight–obese individuals. Cytokine, 77, 1-9. doi: https://doi.org/10.1016/j.cyto.2015.10.003
Glocker, E.-O., Kotlarz, D., Boztug, K., Gertz, E. M., Schäffer, A. A., Noyan, F., . . . Murugan, D. (2009). Inflammatory bowel disease and mutations affecting the interleukin-10 receptor. New England Journal of Medicine, 361(21), 2033-2045. doi: https://doi.org/10.1056/NEJMoa0907206
Hallsworth, K., Thoma, C., Hollingsworth, K. G., Cassidy, S., Anstee, Q. M., Day, C. P., & Trenell, M. I. (2015). Modified high-intensity interval training reduces liver fat and improves cardiac function in non-alcoholic fatty liver disease: a randomized controlled trial. Clinical Science, 129(12), 1097-1105. doi: https://doi.org/10.1042/CS20150308
Jia, D., Cai, M., Xi, Y., & Du, S. (2018). Interval exercise training increases LIF expression and prevents myocardial infarction-induced skeletal muscle atrophy in rats. Life sciences, 193, 77-86. doi: https://doi.org/10.1016/j.lfs.2017.12.009
Kalaki-Jouybari, F., Shanaki, M., Delfan, M., Gorgani-Firouzjae, S., & Khakdan, S. (2020). High-intensity interval training (HIIT) alleviated NAFLD feature via miR-122 induction in liver of high-fat high-fructose diet induced diabetic rats. Archives of physiology and biochemistry, 126(3), 242-249. doi: https://doi.org/10.1080/13813455.2018.1510968
Keating, S. E., Hackett, D. A., Parker, H. M., O’Connor, H. T., Gerofi, J. A., Sainsbury, A., . . . George, J. (2015). Effect of aerobic exercise training dose on liver fat and visceral adiposity. Journal of hepatology, 63(1), 174-182. doi: https://doi.org/10.1016/j.jhep.2015.02.022
Krishnamurthy, P., Rajasingh, J., Lambers, E., Qin, G., Losordo, D. W., & Kishore, R. (2009). IL-10 inhibits inflammation and attenuates left ventricular remodeling after myocardial infarction via activation of STAT3 and suppression of HuR. Circulation research, 104(2), e9-e18. doi: https://doi.org/10.1161/CIRCRESAHA.108.188243
Magrì, S., Paduano, D., Chicco, F., Cingolani, A., Farris, C., Delogu, G., . . . Casula, L. (2019). Nonalcoholic fatty liver disease in patients with inflammatory bowel disease: Beyond the natural history. World Journal of Gastroenterology, 25(37), 5676. doi: https://doi.org/10.3748/wjg.v25.i37.5676
Nicklas, B. J., You, T., & Pahor, M. (2005). Behavioural treatments for chronic systemic inflammation: effects of dietary weight loss and exercise training. Cmaj, 172(9), 1199-1209. doi: https://doi.org/10.1503/cmaj.1040769
Nicoletti, A., Ponziani, F. R., Biolato, M., Valenza, V., Marrone, G., Sganga, G., . . . Grieco, A. (2019). Intestinal permeability in the pathogenesis of liver damage: From non-alcoholic fatty liver disease to liver transplantation. World Journal of Gastroenterology, 25(33), 4814. doi: https://doi.org/10.3748/wjg.v25.i33.4814
Poynard, T., McHutchison, J., Manns, M., Trepo, C., Lindsay, K., Goodman, Z., . . . Albrecht, J. (2002). Impact of pegylated interferon alfa-2b and ribavirin on liver fibrosis in patients with chronic hepatitis C. Gastroenterology, 122(5), 1303-1313. doi: https://doi.org/10.1053/gast.2002.33023
Rodrigues Brandao-Rangel, M. A., Bachi, A. L. L., Oliveira-Junior, M. C., Abbasi, A., Silva-Renno, A., Aparecida de Brito, A., . . . Paula Vieira, R. (2017). Exercise inhibits the effects of smoke-induced COPD involving modulation of STAT3. Oxidative Medicine and Cellular Longevity, 2017. doi: https://doi.org/10.1155/2017/6572714
Roers, A., Siewe, L., Strittmatter, E., Deckert, M., Schlüter, D., Stenzel, W., . . . Müller, W. (2004). T cell–specific inactivation of the interleukin 10 gene in mice results in enhanced T cell responses but normal innate responses to lipopolysaccharide or skin irritation. The Journal of experimental medicine, 200(10), 1289-1297. doi:  https://doi.org/10.1084/jem.20041789
Shabana, M., Ibrahim, H. M., Khadre, S. E., & Elemam, M. G. (2012). Influence of rifampicin and tetracycline administration on some biochemical and histological parameters in albino rats. The Journal of Basic & Applied Zoology, 65(5), 299-308. doi: https://doi.org/10.1016/j.jobaz.2012.10.009
Shafieerad, E., Isanejad, A., & Nasiri, E. (2018). The effects of 8 weeks of endurance exercise training on IL-10 level and body composition of sedentary overweight women. Journal of Practical Studies of Biosciences in Sport, 6(11), 49-57. doi: 10.22077/JPSBS.2018.845
Shaw, D. M., Merien, F., Braakhuis, A., & Dulson, D. (2018). T-cells and their cytokine production: The anti-inflammatory and immunosuppressive effects of strenuous exercise. Cytokine, 104, 136-142. doi: https://doi.org/10.1016/j.cyto.2017.10.001
Vahdat, H., Mombini, H., Eslami Farsani, M., Ab Abzadeh, S., & Barzegar, H. (2018). Effect of High-Intensity Interval Training (HIIT) on the levels of Irisin and interleukin-10 in overweight men. Qom University of Medical Sciences Journal, 12(2), 35-44.
Vickers, N. J. (2017). Animal communication: when i’m calling you, will you answer too? Current Biology, 27(14), R713-R715. doi: https://doi.org/10.1016/j.cub.2017.05.064
 
Journal of Exercise & Organ Cross Talk
Volume 2, Issue 4
December 2022
Pages 150-154

Files

History

  • Receive Date: 25 October 2022
  • Revise Date: 17 December 2022
  • Accept Date: 21 December 2022

Share

How to cite

Statistics