The effect of high intensity interval training and Nano selenium supplementation on the expression of IL-10 and IL-12 genes in testicular tissue in rat under the use of dexamethasone

Document Type : Original Article

Authors

Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.

Abstract

Interval training and selenium is an effective way to strengthen the immune system. The purpose of this research was to investigate the effect of High intensity interval training and Nano selenium supplementation on the expression of IL-10 and IL-12 genes in the testicular tissue of mice under dexamethasone administration. 40 Wistar rats aged 10 weeks were randomly divided into 5 groups of 8 (healthy, dexamethasone induced (patient), High intensity interval training + patient, Nano selenium supplement + patient, High intensity interval training + Nano selenium supplement+ patient). the exercise training program consisted of 5-12 repetitions of 1-minute running on the rodent treadmill with an intensity of 24-30 m/min, equivalent to 75-100% of maximal oxygen consumption, with active rest intervals of 75 seconds. These trainings were performed six days a week for four weeks. The prepared selenium nanoparticle solution, 100 mg in 250 nm size, was given to mice by gavage every other day. One-way analysis of variance was used to determine the difference in variables between groups at a significant level (P<0.05). All statistical calculations were done using SPSS version 25 software. The obtained results showed that interval training and Nano selenium supplementation led to a significant decrease in IL-10 (P<0.05). Also, IL-12 gene expression increased significantly along with interval training and Nano selenium supplementation (P<0.05). According to the current research, it seems that High intensity interval training and Nano selenium supplementation lead to improved immune function and possibly the ability to develop antioxidant defenses and reduce cellular oxidative stress.

What is already known on this subject?

The use of dexamethasone increases the oxidative stress of the testicles and creates a pathological condition and an antioxidant imbalance in the testicular cells. The body's immune cells give a faster and stronger inflammatory response to these conditions causing an increase in IL-10 gene expression in testicular cells. With the presence of IL-10, the expression of interleukin 12 gene decreases because IL-10 is an anti-inflammatory factor.

 

What this study adds?

High intensity interval training and Nano selenium supplementation lead to improved immune function and possibly the ability to develop antioxidant defenses and reduce cellular oxidative stress.

Keywords

Main Subjects


Arabzadeh, E., Shirvani, H., Ebadi Zahmatkesh, M., Riyahi Malayeri, S., Meftahi, G. H., & Rostamkhani, F. (2022). Irisin/FNDC5 influences myogenic markers on skeletal muscle following high and moderate-intensity exercise training in STZ-diabetic rats. 3 Biotech, 12(9), 193. doi: https://doi.org/10.1007/s13205-022-03253-9
Coutinho, A. E., & Chapman, K. E. (2011). The anti-inflammatory and immunosuppressive effects of glucocorticoids, recent developments and mechanistic insights. Mol Cell Endocrinol, 335(1), 2-13. doi: https://doi.org/10.1016/j.mce.2010.04.005
Davoodi, M., Zilaei Bouri, S., & Dehghan Ghahfarokhi, S. (2021). Antioxidant effects of aerobic training and crocin consumption on doxorubicin-induced testicular toxicity in rats. J Family Reprod Health, 15(1), 28-37. doi: https://doi.org/10.18502/jfrh.v15i1.6075
Dehghani, F., Hossieni, S. A., Noorafshan, A., Panjehshahin, M. R., & Esmaeilpour, T. (2021). Effect of selenium on quantitative structural changes in dexamethasone-induced immunodeficiency rat models. Iran J Med Sci, 46(2), 128-135. doi: https://doi.org/10.30476/ijms.2020.81137.0
Dutta, S., Sengupta, P., Slama, P., & Roychoudhury, S. (2021). Oxidative stress, testicular inflammatory pathways, and male reproduction. Int J Mol Sci, 22(18). doi: https://doi.org/10.3390/ijms221810043
Farazandeh Nia, D., Hosseini, M., Riyahi Malayeri, S., & Daneshjoo, A. (2018). Effect of eight weeks of swimming training with garlic intake on serum levels of IL-10 and TNF-α in obese male rats. Jundishapur Scientific Medical Journal, 16(6), 665-671. doi: https://doi.org/10.22118/jsmj.2018.57830
Hajizadeh Maleki, B., & Tartibian, B. (2020). High-intensity interval training modulates male factor infertility through anti-inflammatory and antioxidative mechanisms in infertile men: A randomized controlled trial. Cytokine, 125, 154861. doi: https://doi.org/10.1016/j.cyto.2019.154861
Hamza, T., Barnett, J. B., & Li, B. (2010). Interleukin 12 a key immunoregulatory cytokine in infection applications. Int J Mol Sci, 11(3), 789-806. doi: https://doi.org/10.3390/ijms11030789
Hedayati, S., Riyahi Malayeri, S., & Hoseini, M. (2018). The effect of eight weeks of high and moderate intensity interval training along with aloe vera consumption on serum levels of chemerin, glucose and insulin in streptozotocin-induced diabetic rats: an experimental study. Journal of Rafsanjan University of Medical Sciences, 17(9), 801-814.
Iyer, S. S., & Cheng, G. (2012). Role of interleukin 10 transcriptional regulation in inflammation and autoimmune disease. Crit Rev Immunol, 32(1), 23-63. doi: https://doi.org/10.1615/critrevimmunol.v32.i1.30
Jankovic, D., Kugler, D. G., & Sher, A. (2010). IL-10 production by CD4+ effector T cells: a mechanism for self-regulation. Mucosal Immunol, 3(3), 239-246. doi: https://doi.org/10.1038/mi.2010.8
Kalhor, F., Arshadi, S., & Zafari, A. (2020). The effect of resistance training on Atrogin, eotaxin and IL-10 indices in obese women. Razi Journal of Medical Sciences, 27(3), 130-137.
Liu, J., Cao, S., Kim, S., Chung, E. Y., Homma, Y., Guan, X., . . . Ma, X. (2005). Interleukin-12: an update on its immunological activities, signaling and regulation of gene expression. Curr Immunol Rev, 1(2), 119-137. doi: https://doi.org/10.2174/1573395054065115
Malayeri, S. R., Nikbakht, H., & AliGaeini, A. (2014). Serum chemerin levels and insulin resistance response to highintensity interval training in overweight men. Bulletin of Environment, Pharmacology and Life Sciences, 3(Special Issue II), 385-389.
Mirsepasi, M., Baneifar, A. A., Azarbayjani, M. A., & Arshadi, S. (2019). The effects of high intensity interval training on gene expression of AKT1 and mTORc1 in the left ventricle of type 2 diabetic rats: an experimental study. Journal of Rafsanjan University of Medical Sciences, 17(12), 1119-1130.
Moayeri, P., Kojouri, G., Jafari dehkordi, A., & Ahadi, A. M. (2018). Study of Selenium nanoparticles and sodium selenite supplementation effects on expression of leptin gene in pregnant ewes placenta. Journal of Veterinary Research, 73(4), 475-482. doi: https://doi.org/10.22059/jvr.2019.228023.2592
Mohammadi, S., Rostamkhani, F., Riyahi Malayeri, S., & Shirvani, H. (2022). High-intensity interval training with probiotic supplementation decreases gene expression of NF-κβ and CXCL2 in small intestine of rats with steatosis. Sport Sciences for Health, 18(2), 491-497. doi: https://doi.org/10.1007/s11332-021-00829-5
Mohammadpour, A. A., & Mohammadeini, A. (2021). Protective effect of selenium in prednisolone testicular toxicity. Comparative Clinical Pathology, 30(5), 847-856. doi: https://doi.org/10.1007/s00580-021-03276-3
Nakagawa, T., Zhang, T., Kushi, R., Nakano, S., Endo, T., Nakagawa, M., . . . Nakayama, K. (2017). Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells. Development, 144(22), 4137-4147. doi: https://doi.org/10.1242/dev.158485
Nassan, M. A., Soliman, M. M., Aldhahrani, A., El-Saway, H. B., & Swelum, A. A. (2021). Ameliorative impacts of Allium cepa Linnaeus aqueous extract against testicular damage induced by dexamethasone. Andrologia, 53(4), e13955. doi: https://doi.org/10.1111/and.13955
Noreen, S., Maqbool, I., & Madni, A. (2021). Dexamethasone: Therapeutic potential, risks, and future projection during COVID-19 pandemic. Eur J Pharmacol, 894, 173854. doi: https://doi.org/10.1016/j.ejphar.2021.173854
Orazizadeh, M., Khorsandi, L. S., & Hashemitabar, M. (2010). Toxic effects of dexamethasone on mouse testicular germ cells. Andrologia, 42(4), 247-253. doi: https://doi.org/10.1111/j.1439-0272.2009.00985.x
Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., . . . Bitto, A. (2017). Oxidative Stress: harms and benefits for human health. Oxid Med Cell Longev, 2017, 8416763. doi: https://doi.org/10.1155/2017/8416763
Prihatno, S. A., Adi, Y. K., & Budipitojo, T. (2020). Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome. J Adv Vet Anim Res, 7(3), 514-520. doi: https://doi.org/10.5455/javar.2020.g448
Qazi, I. H., Angel, C., Yang, H., Zoidis, E., Pan, B., Wu, Z., . . . Zhou, G. (2019). Role of selenium and selenoproteins in male reproductive function: a review of past and present evidences. Antioxidants (Basel), 8(8). doi: https://doi.org/10.3390/antiox8080268
Ramamoorthy, S., & Cidlowski, J. A. (2016). Corticosteroids: mechanisms of action in health and disease. Rheum Dis Clin North Am, 42(1), 15-31, vii. doi: https://doi.org/10.1016/j.rdc.2015.08.002
Ramírez-Acosta, S., Selma-Royo, M., Collado, M. C., Navarro-Roldán, F., Abril, N., & García-Barrera, T. (2022). Selenium supplementation influences mice testicular selenoproteins driven by gut microbiota. Sci Rep, 12(1), 4218. doi: https://doi.org/10.1038/s41598-022-08121-3
Riyahi Malayeri, S., Abdolhay, S., Behdari, R., & Hoseini, M. (2019). The combined effect of resveratrol supplement and endurance training on IL-10 and TNF- α in type 2 diabetic rats. Razi Journal of Medical Sciences, 25(12), 140-149.
Riyahi Malayeri, S., Azadniya, A., & Rasaee, M. J. (2019). Effect of eight-week high intensity interval training and resveratrol intake on serum adiponectin and resistin in type 2 diabetic rats. Iranian Journal of Diabetes and Lipid Disorders, 18(1), 8-1.
Riyahi Malayeri, S., & Saei, M. (2019). Changes in Insulin resistance and serum levels of resistin after 10 weeks high intensity interval training in overweight and obese men. Sport Physiology & Management Investigations, 10(4), 31-42.
Soleimani Ghaleh, O., Riyahi Malayeri, S., & Abedi, B. (2022). The effect of interval training and Nano selenium supplementation on the expression of testicular HSP70 and PGC-1α genes in dexamethasone induced rats. Medical Journal of Mashhad University of Medical Sciences, 65(2), -. doi: https://doi.org/10.22038/mjms.2022.21339
Steen, E. H., Wang, X., Balaji, S., Butte, M. J., Bollyky, P. L., & Keswani, S. G. (2020). The Role of the anti-inflammatory cytokine interleukin-10 in tissue fibrosis. Adv Wound Care (New Rochelle), 9(4), 184-198. doi: https://doi.org/10.1089/wound.2019.1032
Taherkhani, S., Suzuki, K., & Castell, L. (2020). A short overview of changes in inflammatory cytokines and oxidative stress in response to physical activity and antioxidant supplementation. Antioxidants (Basel), 9(9). doi: https://doi.org/10.3390/antiox9090886
Tugues, S., Burkhard, S. H., Ohs, I., Vrohlings, M., Nussbaum, K., Vom Berg, J., . . . Becher, B. (2015). New insights into IL-12-mediated tumor suppression. Cell Death Differ, 22(2), 237-246. doi: https://doi.org/10.1038/cdd.2014.134