The effect of high intensity interval training and Nano selenium supplementation on the expression of IL-10 and IL-12 genes in testicular tissue in rat under the use of dexamethasone

Document Type : Original Article


Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.


Interval training and selenium is an effective way to strengthen the immune system. The purpose of this research was to investigate the effect of High intensity interval training and Nano selenium supplementation on the expression of IL-10 and IL-12 genes in the testicular tissue of mice under dexamethasone administration. 40 Wistar rats aged 10 weeks were randomly divided into 5 groups of 8 (healthy, dexamethasone induced (patient), High intensity interval training + patient, Nano selenium supplement + patient, High intensity interval training + Nano selenium supplement+ patient). the exercise training program consisted of 5-12 repetitions of 1-minute running on the rodent treadmill with an intensity of 24-30 m/min, equivalent to 75-100% of maximal oxygen consumption, with active rest intervals of 75 seconds. These trainings were performed six days a week for four weeks. The prepared selenium nanoparticle solution, 100 mg in 250 nm size, was given to mice by gavage every other day. One-way analysis of variance was used to determine the difference in variables between groups at a significant level (P<0.05). All statistical calculations were done using SPSS version 25 software. The obtained results showed that interval training and Nano selenium supplementation led to a significant decrease in IL-10 (P<0.05). Also, IL-12 gene expression increased significantly along with interval training and Nano selenium supplementation (P<0.05). According to the current research, it seems that High intensity interval training and Nano selenium supplementation lead to improved immune function and possibly the ability to develop antioxidant defenses and reduce cellular oxidative stress.

What is already known on this subject?

The use of dexamethasone increases the oxidative stress of the testicles and creates a pathological condition and an antioxidant imbalance in the testicular cells. The body's immune cells give a faster and stronger inflammatory response to these conditions causing an increase in IL-10 gene expression in testicular cells. With the presence of IL-10, the expression of interleukin 12 gene decreases because IL-10 is an anti-inflammatory factor.


What this study adds?

High intensity interval training and Nano selenium supplementation lead to improved immune function and possibly the ability to develop antioxidant defenses and reduce cellular oxidative stress.


Main Subjects

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