The effect of eight weeks of interval and continuous endurance training on PGC-1α and FNDC5 gene expression in the gastrocnemius muscle of male Wistar rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Department of Basic Sciences and Hygine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Associate Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

4 Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

10.22034/jeoct.2022.363215.1053

Abstract

The purpose of this study was to investigate and compare the effect of eight weeks of continuous and interval training on PGC-1α and FNDC5 gene expression in the gastrocnemius muscle of male Wistar rats. 30 eight-week-old rats with an average weight of 192 ± 10 were randomly divided into 3 groups (n=10): control group (Ctrl), interval endurance training group (IET) and continuous endurance training group (CET). The exercise groups participated in 8 weeks of continuous or interval training program (5 days per week). 24 hours after the last training session, all the rats were anesthetized and their gastrocnemius muscles were collected and transferred to the laboratory to measure the mRNA levels of PGC-1α and FNDC5 genes by Real Time-PCR method. The results showed that the expression level of FNDC5 in the gastrocnemius muscle was significantly higher in the interval and continuous endurance training groups than in the control group. Also, the expression of this gene was higher in the interval group than in the continuous group. It was also found that the expression of PGC-1α gene was significantly increased in both training groups compared to the control group. Also, the expression of this gene increased significantly in the interval training group compared to the continuous. It seems that interval endurance training has a greater effect on the expression of PGC-1α and FNDC5 factors in the gastrocnemius muscle.

What is already known on this subject?

Although PGC-1α plays an important role in regulating cellular energy metabolism, it is most likely involved in metabolic disorders such as obesity, diabetes, and cardiomyopathy.

 

What this study adds?

Interval endurance training in the gastrocnemius muscle of Wistar rats increases the expression of PGC-1α mRNA and subsequently FNDC5 mRNA more than continuous endurance training.

Keywords

Main Subjects


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Volume 2, Issue 3
September 2022
Pages 91-95
  • Receive Date: 29 May 2022
  • Revise Date: 16 September 2022
  • Accept Date: 22 September 2022
  • First Publish Date: 22 September 2022