Determining the range of aerobic exercise on a treadmill for male Wistar rats at different ages: A pilot study

Document Type : Short Communication

Authors

1 Assistant Professor, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.

2 Ph.D. Candidate of exercise physiology, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.

3 Associate Professor, Department of physical Education and sport science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.

4 Baqiyatallah University of Medical Science.

Abstract

The purpose of this study was to determining the range of aerobic exercise on a treadmill for male Wistar rats at different ages. Twelve male Wistar rats were divide in three groups of immature, adults, and old (n= 4 in each). At first session, the rats began to run at a rate of 2 m/min to perform the fatigue test, and the treadmill speed was increased by 2 m/min every 2 minutes. This process of acceleration continued until the rats were no longer able to continue moving on the treadmill and became exhausted. Then, blood lactate of each subject measured immediately and their maximum speed was recorded. After 48 hours of recovery, animal performed maximum recorded speed on a treadmill in three 10-minute steps of 25%, 50% and 75%, respectively. Immediately after each percent blood lactate were measured and recorded. Immature rats at an average speed of 18 m/min were reached to their maximum speed with an average lactate concentration of 8±1.8 mmol/l. Adults rats at an average speed of 36 m/min were reached to their maximum speed with an average lactate concentration of 6.8±0.4 mmol/l. The old rats reached their maximum velocity with an average of 30 m/min with an average lactate concentration of 6.95±0.9 mmol/l. Therefore, it recommended that aerobic exercise in untrained rats start at a light speed, i.e. 25% of their maximum speed, which is lower than the lactate threshold, and gradually continue up to 50% of their maximum speed.

What is already known on this subject?

The use of blood lactate can be a good predictor parameter for determining the aerobic range relative to other variables.

 

What this study adds?

The present study provided appropriate data for determining the range of aerobic exercise intensity of immature, adult, and old male rats.

Keywords

Main Subjects


Beneke, R., Leithäuser, R. M., & Ochentel, O. (2011). Blood lactate diagnostics in exercise testing and training. International Journal of Sports Physiology and Performance, 6(1), 8-24. doi: https://doi.org/10.1123/ijspp.6.1.8
Booth, F. W., Laye, M. J., & Spangenburg, E. E. (2010). Gold standards for scientists who are conducting animal-based exercise studies. Journal of Applied Physiology, 108(1), 219-221. doi: https://doi.org/10.1152/japplphysiol.00125.2009
Brito Vieira, W., Halsberghe, M., Schwantes, M., Perez, S., Baldissera, V., Prestes, J., . . . Parizotto, N. (2014). Increased lactate threshold after five weeks of treadmill aerobic training in rats. Brazilian Journal of Biology, 74, 444-449. doi: https://doi.org/10.1590/1519-6984.er7403
Carvalho, J., Masuda, M.O., & Pompeu, F.A. (2005). Method for diagnosis and control of aerobic training in rats based on lactate threshold. Comp Biochem Physiol A Mol Integr Physiol, 140, 409-413. doi: https://doi.org/10.1016/j.cbpb.2004.12.002
Christensen, S., Mikkelsen, L., Fels, J., Bodvarsdottir, T., & Hansen, A. (2009). Quality of plasma sampled by different methods for multiple blood sampling in mice. Laboratory Animals, 43(1), 65-71. doi: https://doi.org/10.1258/la.2008.007075
Contarteze, R. V. L., Manchado, F. D. B., Gobatto, C. A., & De Mello, M. A. R. (2008). Stress biomarkers in rats submitted to swimming and treadmill running exercises. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 151(3), 415-422. doi: https://doi.org/10.1016/j.cbpa.2007.03.005
Faude, O., Kindermann, W., & Meyer, T. (2009). Lactate threshold concepts. Sports Medicine, 39(6), 469-490. doi: https://doi.org/10.2165/00007256-200939060-00003
Gitt, A. K., Wasserman, K., Kilkowski, C., Kleemann, T., Kilkowski, A., Bangert, M., . . . Senges, J. (2002). Exercise anaerobic threshold and ventilatory efficiency identify heart failure patients for high risk of early death. Circulation, 106(24), 3079-3084. doi: https://doi.org/10.1161/01.CIR.0000041428.99427.06
Gonzalez, N. C., Clancy, R. L., Moue, Y., & Richalet, J. P. (1998). Increasing maximal heart rate increases maximal O2 uptake in rats acclimatized to simulated altitude. Journal of Applied Physiology, 84(1), 164-168. doi: https://doi.org/10.1152/jappl.1998.84.1.164
Kawanishi, N., Takagi, K., Lee, H.-C., Nakano, D., Okuno, T., Yokomizo, T., & Machida, S. (2018). Endurance exercise training and high-fat diet differentially affect composition of diacylglycerol molecular species in rat skeletal muscle. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 314(6), R892-R901. doi: https://doi.org/10.1152/ajpregu.00371.2017
Kumagai, S., & Nishizumi, M. (1986). Evaluation of exercise intensity indicated by blood lactate in rats during treadmill exercise. Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene), 41(3), 648-652. doi: https://doi.org/10.1265/jjh.41.648
Manchado-Gobatto, F., Gobatto, C., Contarteze, R., & Mello, M. (2011). Non-exhaustive test for aerobic capacity determination in running rats.
Shepherd, R. E., & Gollnick, P. D. (1976). Oxygen uptake of rats at different work intensities. Pflügers Archiv, 362(3), 219-222. doi: https://doi.org/10.1007/BF00581173
Tsumiyama, W., Oki, S., Tamaru, M., Ono, T., Shimizu, M. E., & Otsuka, A. (2012). Evaluation of the lactate threshold of rats using external jugular vein catheterization. Journal of Physical Therapy Science, 24(11), 1107-1109. doi: https://doi.org/10.1589/jpts.24.1107
Voltarelli, F., Gobatto, C., & De Mello, M. (2002). Determination of anaerobic threshold in rats using the lactate minimum test. Brazilian Journal of Medical and Biological Research, 35, 1389-1394. doi: https://doi.org/10.1590/S0100-879X2002001100018