Moderate-intensity continuous training and probiotic consumption on IL-15 gene expression in an animal model of non-alcoholic steatosis

Document Type : Original Article

Authors

Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.

10.22034/jeoct.2022.351857.1048

Abstract

Steatosis is the most important cause for chronic liver disease, from simple steatosis to advanced stages such as liver fibrosis and cirrhosis and liver cancer. In this study, the effect of MICT and probiotic consumption on IL15 gene expression in animal model of non-alcoholic steatosis was examined. This study is an experimental laboratory study. Thirty-two male wistar rats were divided into 4 groups of 8 in the healthy (normal diet), steatosis, steatosis + probiotic, steatosis + probiotic +MICT groups and tested for 8 weeks. The exercise protocol was as follows: in the first week from 10 minutes of running at a speed of 18 meters per minute to the eighth week with 60 minutes of running at a speed of 28 meters per minute. Consumption of probiotics; Relevant groups received 109 CFU / ml of Lactobacillus rhamnosus GG by gavage daily for 5 weeks and 5 days a week. Statistical calculation of this study was performed using SPSS 25 software. Probiotic consumption and moderate-intensity continuous training significantly increased IL-15 (p =0.000) and significantly decreased TG (p =0.000) in the non-alcoholic steatosis animal model. It seems moderate-intensity continuous training with probiotic consumption can improve the liver function of non-alcoholic fatty liver patients.

What is already known on this subject?

Although the number of studies on IL15 measures has increased in the last years, to date, it remains an open question whether the existing effect of exercise and probiotic consumption on immune system measures are appropriate for non-alcoholic fatty liver.

 

What this study adds?

Moderate-intensity continuous training with probiotic consumption can improve the liver function of non-alcoholic fatty liver patients

Keywords

Main Subjects


Alves-Bezerra, M., & Cohen, D. E. (2017). Triglyceride metabolism in the liver. Compr Physiol, 8(1), 1-8. doi: https://doi.org/10.1002/cphy.c170012
As’Habi, A., Nazari, M., Hajianfar, H., Arab, A., & Faghfoori, Z. (2020). A systematic review and meta-analysis of probiotic consumption and metabolic status of athletes. International Journal of Food Properties, 23(1), 941-954. doi: https://doi.org/10.1080/10942912.2020.1772286
Asgari Hazaveh, D., Riyahi Malayeri, S., & Babaei, S. (2018). Effect of eight weeks high intensity interval training and medium intensity interval training and aloe vera intake on serum vaspin and insulin resistance in diabetic male rats. Journal of Arak University of Medical Sciences, 20(11), 67-75.
Browne, H. P., Neville, B. A., Forster, S. C., & Lawley, T. D. (2017). Transmission of the gut microbiota: Spreading of health. Nat Rev Microbiol, 15(9), 531-543. doi: https://doi.org/10.1038/nrmicro.2017.50
Cavallini, D. C. U., Suzuki, J. Y., Abdalla, D. S. P., Vendramini, R. C., Pauly-Silveira, N. D., Roselino, M. N., . . . Rossi, E. A. (2011). Influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in an animal model. Lipids in Health and Disease, 10(1), 126. doi: https://doi.org/10.1186/1476-511X-10-126
Cepero-Donates, Y., Lacraz, G., Ghobadi, F., Rakotoarivelo, V., Orkhis, S., Mayhue, M., . . . Ramanathan, S. (2016). Interleukin-15-mediated inflammation promotes non-alcoholic fatty liver disease. Cytokine, 82, 102-111. doi: https://doi.org/10.1016/j.cyto.2016.01.020
Hedayati, S., Riyahi Malayeri, S., & Hoseini, M. (2018). The effect of eight weeks of high and moderate intensity interval training along with aloe vera consumption on serum levels of chemerin, glucose and insulin in streptozotocin-induced diabetic rats: An experimental study. Journal of Rafsanjan University of Medical Sciences, 17(9), 801-814.
Hosseini, M., Eftekhar, B., & Riyahi Malayeri, S. (2017). Effect of interval training with curcumin consumption on some adipokines in menopausal obese rats. Journal of Rafsanjan University of Medical Sciences, 16(6), 505-516.
Jeong, J. J., Park, H. J., Cha, M. G., Park, E., Won, S. M., Ganesan, R., . . . Suk, K. T. (2022). The lactobacillus as a probiotic: Focusing on liver diseases. Microorganisms, 10(2). doi: https://doi.org/10.3390/microorganisms10020288
Leal, L. G., Lopes, M. A., & Batista, M. L. (2018). Physical exercise-induced myokines and muscle-adipose tissue crosstalk: A review of current knowledge and the implications for health and metabolic diseases. Frontiers in Physiology, 9. doi: https://doi.org/10.3389/fphys.2018.01307
Leung, C., Rivera, L., Furness, J. B., & Angus, P. W. (2016). The role of the gut microbiota in NAFLD. Nat Rev Gastroenterol Hepatol, 13(7), 412-425. doi: https://doi.org/10.1038/nrgastro.2016.85
Li, L., Liu, H., Hu, X., Huang, Y., Wang, Y., He, Y., & Lei, Q. (2018). Identification of key genes in non‑alcoholic fatty liver disease progression based on bioinformatics analysis. Mol Med Rep, 17(6), 7708-7720. doi: https://doi.org/10.3892/mmr.2018.8852
Markowiak, P., & Śliżewska, K. (2017). Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients, 9(9). doi: https://doi.org/10.3390/nu9091021
Mohammadi, S., Rostamkhani, F., Riyahi Malayeri, S., & Shirvani, H. (2021). High-intensity interval training with probiotic supplementation decreases gene expression of NF-κβ and CXCL2 in small intestine of rats with steatosis. Sport Sciences for Health. doi: https://doi.org/10.1007/s11332-021-00829-5
Moslehi, A., & Hamidi-Zad, Z. (2018). Role of SREBPs in liver diseases: A mini-review. J Clin Transl Hepatol, 6(3), 332-338. doi: https://doi.org/10.14218/jcth.2017.00061
Nadeau, L., & Aguer, C. (2019). Interleukin-15 as a myokine: Mechanistic insight into its effect on skeletal muscle metabolism. Appl Physiol Nutr Metab, 44(3), 229-238. doi: https://doi.org/10.1139/apnm-2018-0022
Ofoghi, M., Isanejad, A., & Samadi, A. (2018). Investigating the effect of eight-weeks resistance training with Thera-band on the serum levels of IL-15, balance and muscle strength in elderly women. Metabolism and Exercise, 8(1), 43-60. doi: https://doi.org/10.22124/jme.2018.3563
Parthasarathy, G., Revelo, X., & Malhi, H. (2020). Pathogenesis of nonalcoholic steatohepatitis: An overview. Hepatol Commun, 4(4), 478-492. doi: https://doi.org/10.1002/hep4.1479
Parvez, S., Malik, K. A., Ah Kang, S., & Kim, H. Y. (2006). Probiotics and their fermented food products are beneficial for health. J Appl Microbiol, 100(6), 1171-1185. doi: https://doi.org/10.1111/j.1365-2672.2006.02963.x
Patel, A. K., Singhania, R. R., Pandey, A., & Chincholkar, S. B. (2010). Probiotic bile salt hydrolase: Current developments and perspectives. Appl Biochem Biotechnol, 162(1), 166-180. doi: https://doi.org/10.1007/s12010-009-8738-1
Pei, K., Gui, T., Kan, D., Feng, H., Jin, Y., Yang, Y., . . . Li, Y. (2020). An overview of lipid metabolism and nonalcoholic fatty liver disease. Biomed Res Int, 2020, 4020249. doi: https://doi.org/10.1155/2020/4020249
Soleimany, M., Moazamian, E., & Rasouli, M. (2018). Effect of bacillus thuringiensis parasporal toxin on stimulating of IL-2 and IL-5 cytokines production. Biological Journal of Microorganism, 7(25 #M005).
Thyfault, J. P., & Rector, R. S. (2020). Exercise combats hepatic steatosis: Potential mechanisms and clinical implications. Diabetes, 69(4), 517-524. doi: https://doi.org/10.2337/dbi18-0043
Tolouei Azar, J., Shabkhiz, F., & Khalafi, M. (2021). The effects of eight weeks of resistance training on serum levels IL-15, IL-6, TNF-α and insulin resistance in older type 2 diabetic men. Journal of Sport Biosciences, 12(4), 391-406. doi: https://doi.org/10.22059/jsb.2018.251040.1241
van der Windt, D. J., Sud, V., Zhang, H., Tsung, A., & Huang, H. (2018). The effects of physical exercise on fatty liver disease. Gene Expr, 18(2), 89-101. doi: https://doi.org/10.3727/105221617x15124844266408
Wang, Y., Kirpich, I., Liu, Y., Ma, Z., Barve, S., McClain, C. J., & Feng, W. (2011). Lactobacillus rhamnosus GG treatment potentiates intestinal hypoxia-inducible factor, promotes intestinal integrity and ameliorates alcohol-induced liver injury. Am J Pathol, 179(6), 2866-2875. doi: https://doi.org/10.1016/j.ajpath.2011.08.039
Zhang, X., Cao, L., Ji, B., Li, L., Qi, Z., & Ding, S. (2020). Endurance training but not high-intensity interval training reduces liver carcinogenesis in mice with hepatocellular carcinogen diethylnitrosamine. Exp Gerontol, 133, 110853. doi: https://doi.org/10.1016/j.exger.2020.110853
 
 
 
 
Volume 2, Issue 3
September 2022
Pages 85-90
  • Receive Date: 01 May 2022
  • Revise Date: 06 July 2022
  • Accept Date: 16 July 2022
  • First Publish Date: 16 July 2022