Influence of high intensity interval training on adipose tissue PAI-2 and MMP-2 mRNAs expression in rat with high-fat diet-induced metabolic syndrome

Document Type : Original Article


1 Student of Ph.D of Physical Education and Sport Sciences, Departement of Physical Education and Sport Sciences, Faculty of Education and Psychology, University of Mohagheghe Ardabili, Ardabil, Iran.

2 Department of Sport Physiology, Faculty of Physical Education, Islamic Azad University, Tehran, Iran.

3 Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran


The aim of this study was to investigate the influence of high intensity interval training on adipose tissue PAI-2 and MMP-2 mRNAs expression in rat with high-fat diet-induced metabolic syndrome. In this experimental study, 32 male Wistar rats (120 to 130 g) were selected and after 12 weeks of high-fat diet and modeling of metabolic syndrome were randomly divided into 4 groups: control, metabolic syndrome (Mets), high intensity interval training (HIIT) and Mets + HIIT. The HIIT program included 5 to 10 intense running on the treadmill with an intensity of 80 to 95% of the maximum speed and in slow running with a speed of 55% of the maximum speed with a zero slope of the treadmill for 8 weeks. Data were analyzed using one-way analysis of variance (ANOVA). The results of one-way analysis of variance showed that there was a difference between the mean expression of MMP-2 and PAI-2 mRNA in adipose tissue of male rats with metabolic syndrome in different groups (P = 0.001). However, HIIT improve this gene after Mets in adipose tissue (p<0.05). It seems that HIIT program improve metabolic syndrome with controlling PAI-2 mRNA and have an anti-inflammatory effect in adipose tissue. However, it need to more studies especially on human sample.

What is already known on this subject?

Obesity can lead to increased inflammatory status due to dysregulation of anti-inflammatory adipokines such as decreased adiponectin and increased pro-inflammatory factors including PAI-1.


What this study adds?

HIIT exercise with negative regulation of PAI-2 and positive regulation of MMP-2 has a beneficial role on adipose tissue.


Main Subjects

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