The effect of high-intensity interval swimming training on ULK and TSC1/2 proteins of hippocampus tissue in elderly rats

Document Type : Original Article


Department of Physical Education and Sport Sciences, Yadegar-e-Imam Khomeini (RAH) Shahr-e Ray Branch, Islamic Azad University, Tehran, Iran


Understanding the cellular and molecular mechanisms that regulate aging reduces the possibility of dependence on age-related diseases. Exercise treatment strategies reduce the burden of aging-related illness, disability, and premature death in the elderly. The aim of this study was to evaluate the effect of high-intensity interval swimming training on ULK and TSC1/2 proteins of hippocampus tissue in elderly rats. In this experimental study, 16 elderly male Sprague Dawley rats (20 months old and mean weight 300-450 g) were divided into two groups of control and high-intensity interval swimming training. High-intensity interval swimming training consisted of 14 bouts 20-second swimming sessions with 10 seconds of rest between each session for six weeks (three days a week). The content of ULK and TSC1/2 proteins in hippocampal tissue was measured by Western blotting. Data were analyzed using independent t-test at the P<0.05. The results showed that high-intensity interval swimming training caused significant increase in content of ULK protein of hippocampus tissue in elderly rats (P=0.010). Also, high-intensity interval swimming training caused significant decrease in content of TSC1/2 protein of hippocampus tissue in elderly rats (P=0.010). According to the results, it seems that high-intensity interval swimming training can help improve the homeostasis regulatory pathways in hippocampal neurons in the elderly animal model.

What is already known on this subject?

Most research on HIIT has been performed in young and middle-aged adults and, therefore, its tolerance and effects are less well known in the elderly.


What this study adds?

Six weeks of high-intensity intermittent swimming training resulted in a significant increase in ULK protein content and a significant decrease in hippocampal tissue TSC1/2 protein content in elderly rats.


Main Subjects

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Volume 2, Issue 1
March 2022
Pages 22-27
  • Receive Date: 27 January 2022
  • Revise Date: 10 March 2022
  • Accept Date: 19 March 2022
  • First Publish Date: 19 March 2022