The effect of 12 weeks of aerobic exercise intervention on bone mineral density, expression of lymphocyte alkaline phosphatase gene and bone turnover markers in overweight postmenopausal women: a randomized controlled trial

Document Type : Original Article

Authors

1 Ph.D of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.

2 Head of Exercise Physiology Clinic, Urmia, Iran.

3 MSc of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.

4 Full Professor of Exercise Physiology, Department of Sport Injuries & Corrective Exercises, Faculty of Sport Sciences, Allameh Tabataba'i University, Tehran, Iran.

5 Full Professor of Clinical Biochemistry, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

The aim of our study was to examine the effect of 12 weeks of moderate-intensity aerobic exercise on bone mineral density (BMD), lymphocyte alkaline phosphatase (ALP) mRNA expression, and biochemical markers of bone turnover in postmenopausal women (PMWs). Twenty-four healthy sedentary PMWs aged 50-60 years were randomly assigned to exercise (EX, n=12) and control (C, n=12) groups. The EX group performed walking/jogging (50-60min/day, 3days/week at 65%-70% HRmax reserve) for 12-week while the C group participated in no intervention and continued their normal lifestyle. The BMD and lymphocyte ALP mRNA were determined by DXA and qRT-PCR, respectively. After 12 weeks, the increase in the lymphocyte ALP mRNA expression and its serum (P=0.008 and P=0.001), PTH (P=0.001), Vit-D (P=0.002), and VO2max (P=0.001) were significantly higher in the EX group compared to the C group, whereas body fat was significantly decreased (P=0.028). Our study indicates that 12 weeks of moderate-intensity aerobic exercise intervention improves bone turnover by increasing the ALP mRNA expression, serum levels of PTH, ALP, and Vit-D which can lead to the prevention of aging-induced osteopenia among PMWs.

What is already known on this subject?

Studies have examined the effects of exercise on serum markers of bone metabolism and BMD, but their results are completely contradictory.

 

What this study adds?

Twelve weeks of moderate-intensity aerobic exercise intervention improve bone turnover by increasing the ALP mRNA expression, serum levels of PTH, ALP, and Vit-D which can lead to the prevention of aging-induced osteopenia among PMWs.

Keywords

Main Subjects

References

Azadpour, N., Tartibian, B., & Koşar, Ş.N. (2016) Effects of aerobic exercise training on ACE and ADRB2 gene expression, plasma angiotensin II level, and flow-mediated dilation: a study on obese postmenopausal women with prehypertension. Menopause, 24, 269-277. URL: https://pubmed.ncbi.nlm.nih.gov/28231078/
Bailey, R.L., Miller, P.E., Mitchell, D.C., Hartman, T.J., Lawrence, F.R., Sempos, C.T., & Smiciklas-Wright, H. (2009). Dietary screening tool identifies nutritional risk in older adults. The American Journal of Clinical Nutrition, 90, 177-83. PubMed doi: https://doi.org/10.3945/ajcn.2008.27268
    Berridge, M.J. (2016). Vitamin D, reactive oxygen species and calcium signaling in ageing and disease. Philosophical Transactions of the Royal Society of London, Series B, Biological Sciences, 371, 20150434. PubMed doi: https://doi.org/10.1098/rstb.2015.0434
Boonen, S., Dejaeger, E., Vanderschueren, D., Venken, K., Bogaerts, A., Verschueren, S., & Milisen, K. (2008). Osteoporosis and osteoporotic fracture occurrence and prevention in the elderly: a geriatric perspective. Best Practice & Research Clinical Endocrinology & Metabolism, 22, 765-85. PubMed doi: https://doi.org/10.1016/j.beem.2008.07.002
    Cauley, J.A., Danielson, M.E., Greendale, G.A., Finkelstein, J.S., Chang, Y.F., Lo, J.C., . . . Sowers, M.R. (2012). Bone resorption and fracture across the menopausal transition: The Study of Women's Health Across the Nation. Menopause, 19, 1200-1207. PubMed doi: https://doi.org/10.1097/gme.0b013e31825ae17e
   Cerdá Gabaroi, D., Peris, P., Monegal, A., Albaladejo, C., Martínez, M.A., Muxí, A., . . . Guañabens N. (2010). Search for hidden secondary causes in postmenopausal women with osteoporosis. Menopause, 17, 135-9. PubMed doi: https://doi.org/10.1097/gme.0b013e3181ade8e5
      Datta, N.S., & Abou-Samra, A.B. (2009). PTH and PTHrP signaling in osteoblasts. Cellular Signaling, 21, 1245-54. PubMed doi: https://doi.org/10.1016/j.cellsig.2009.02.012
Decuypere, J.P., Monaco, G., Missiaen, L., DeSmedt, H., Parys, J.B., & Bultynck, G. (2011) IP3 receptors, mitochondria, and Ca2+ signaling: implications for aging. Journal of Aging Research, 2011, 1-20. PubMed doi: https://doi.org/10.4061/2011/920178
DeFina, L.F., Leonard, D., Willis, B.L., Barlow, C.E., Finley, C.E., Jenkins, M.R., . . . Shen, C.L. (2016). High cardiorespiratory fitness is associated with reduced risk of low bone density in postmenopausal women. Journal of Women's Health (Larchmt), 25, 1073-1080. PubMed doi: https://doi.org/10.1089/jwh.2014.5170
Eastell, R., Rosen, C.J., Black, D.M., Cheung, A.M., Murad, M.H., & Shoback, D. (2019). Pharmacological management of osteoporosis in postmenopausal women: An endocrine society* clinical practice guideline. The Journal of Clinical Endocrinology & Metabolism, 104, 1595–1622. PubMed doi: https://doi.org/10.1210/jc.2019-00221
Edwards, B.J. (2017). Osteoporosis risk calculators. Journal of Clinical Densitometry, 20, 379-388. PubMed doi: https://doi.org/10.1016/j.jocd.2017.06.024
Fukuda, T., Bouchi, R., Takeuchi, T., Tsujimoto, K., Minami, I., Yoshimoto, T., & Ogawa, Y. (2018). Sarcopenic obesity assessed using dual energy X-ray absorptiometry (DXA) can predict cardiovascular disease in patients with type 2 diabetes: a retrospective observational study. Cardiovascular Diabetology, 17, 55. PubMed doi: https://doi.org/10.1186/s12933-018-0700-5
Goseki-Sone, M., Sogabe, N., Fukushi-Irie, M., Mizoi, L., Orimo, H., Suzuki, T., . . . Hosoi, T. (2005). Functional analysis of the single nucleotide polymorphism (787T>C) in the tissue-nonspecific alkaline phosphatase gene associated with BMD. Journal of Bone and Mineral Research, 20, 773-82. PubMed doi: https://doi.org/10.1359/JBMR.041229
Jilka, R.L., Weinstein, R.S., Bellido, T., Roberson, P., Parfitt, A.M., & Manolagas, S.C. (1999). Increased bone formation by prevention of osteoblast apoptosis with parathyroid hormone. Journal of Clinical Investigation, 104, 439-46. PubMed doi: https://doi.org/10.1172/JCI6610
Karinkanta, S. (2017) Exercise: a comprehensive tool for ageing women to take care of their health. Menopause, 24, 7-8. PubMed doi: https://doi.org/10.1097/GME.0000000000000799
Khalafi, M., Malandish, A., Rosenkranz, S.K (2021). The impact of exercise training on inflammatory markers in postmenopausal women: A systemic review and meta-analysis. Exp Gerontol, 150, 111398. doi: https://doi.org/10.1016/j.exger.2021.111398
Lee, I., Cho, J., Jin, Y., Ha, C., Kim, T., & Kang, H. (2016). Body fat and physical activity modulate the association between sarcopenia and osteoporosis in elderly korean women. Journal of Sports Science and Medicine, 15, 477-482. URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974860/
Livak, K.J., & Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25, 402-8. PubMed doi: https://doi.org/10.1006/meth.2001.1262
Maehata, Y., Takamizawa, S., Ozawa, S., Kato, Y., Sato, S., Kubota, E., & Hata, R. (2006). Both direct and collagen-mediated signals are required for active vitamin D3-elicited differentiation of human osteoblastic cells: roles of osterix, an osteoblast-related transcription factor. Matrix Biology, 25, 47-58. PubMed doi: https://doi.org/10.1016/j.matbio.2005.09.001
Marawan, A., Kurbanova, N., & Qayyum, R. (2018). Association between serum vitamin D levels (VD) and cardiorespiratory fitness in the adult population of the United States. Journal of the American College of Cardiology, 71, A1798. PubMed doi: https://doi.org/10.1016/S0735-1097(18)32339-8
Marawan, A., Kurbanova, N., & Qayyum, R. (2019). Association between serum vitamin D levels and cardiorespiratory fitness in the adult population of the USA. European Journal of Preventive Cardiology, 26, 750-755. PubMed doi: https://doi.org/10.1177/2047487318807279
Malandish, A., Tartibian, B., Rahmati, M., Afsargharehbagh, R., Sheikhlou, Z (2020a). The effect of moderate-intensity aerobic training on pulmonary function and estrogen receptor-alpha gene in postmenopausal women with vitamin D deficiency: A randomized control trial. Respir Physiol Neurobiol, 281, 103510. doi: https://doi.org/10.1016/j.resp.2020.103510
Malandish, A., Tartibian, B., Rahmati Yamchi, M. (2016). Effect of 12 Weeks of Moderate-Intensity Aerobic Training on Bone Density and Serum Indices of Bone in Sedentary Postmenopausal Women. Sport Physiol, 8(32), 67-84. doi: https://doi.org/10.22089/spj.2016.862
Malandish, A., Tartibian, B., Sheikhlou, Z., Afsargharehbagh, R., Rahmati, M (2020b). The effects of short-term moderate intensity aerobic exercise and long-term detraining on electrocardiogram indices and cardiac biomarkers in postmenopausal women. J Electrocardiol, 60, 15-22. doi: https://doi.org/10.1016/j.jelectrocard.2020.03.004
McKinney, J., Lithwick, D.J., Morrison, B.N., Nazzari, H., Isserow, S.H., Heilbron, B., & Krahn, A. (2016). The health benefits of physical activity and cardiorespiratory fitness. BCMedical Journal, 58, 131-137. URL: https://bcmj.org/articles/health-benefits-physical-activity-and-cardiorespiratory-fitness
Mukherjee, A., & Rotwein, P. (2009). Akt promotes BMP2-mediated osteoblast differentiation and bone development. Journal of Cell Science, 122, 716-26. PubMed doi: https://doi.org/10.1242/jcs.042770
Nabipour, I., Larijani, B., Beigi, S., Jafari, S.M., Amiri, M., Assadi, M., . . . Sanjdideh Z. (2008). Relationship among insulinlike growth factor I concentrations, bone mineral density, and biochemical markers of bone turnover in postmenopausal women: a population-based study. Menopause, 15, 934-9. PubMed   doi: https://doi.org/10.1097/gme.0b013e31816665a7
Paredes R, Arriagada G, Cruzat F, Villagra A, Olate J, Zaidi K, . . . Montecino M. (2004). Bone-specific transcription factor Runx2 interacts with the 1alpha,25-dihydroxyvitamin D3 receptor to up-regulate rat osteocalcin gene expression in osteoblastic cells. Molecular and Cellular Biology, 42, 8847-61. PubMed doi: https://doi.org/10.1128/MCB.24.20.8847-8861.2004
Peppel, J.v., & van Leeuwen, J. (2014). Vitamin D and gene networks in human osteoblasts. Frontiers in Physiology, 5, 137. PubMed doi: https://doi.org/10.3389/fphys.2014.00137
Rey, A., Manen, D., Rizzoli, R., Ferrari, S.L., & Caverzasio, J. (2007). Evidences for a role of p38 MAP kinase in the stimulation of alkaline phosphatase and matrix mineralization induced by parathyroid hormone in osteoblastic cells. Bone, 41, 59-67. PubMed doi: https://doi.org/10.1016/j.bone.2007.02.031
Saremi, M.A., Saremi, M., & Tavallaei, M. (2008). Rapid genomic DNA extraction (RGDE). Forensic Science International: Genetics Supplement Series, 1, 63-65. PubMed doi: https://doi.org/10.1016/j.fsigss.2007.12.001
Sharma, U., Pal, D., & Prasad, R. (2014). Alkaline Phosphatase: An overview. Indian Journal of Clinical Biochemistry, 29, 269-278. PubMed doi: https://doi.org/10.1007/s12291-013-0408-y
Sheikhlou, Z., Tartibian, B., Malandish, A (2016). Effect of 12 weeks of moderate-intensity aerobic exercise (W-WJMIEP-R) on serum levels of vitamin D, parathyroid hormone, calcium, and phosphorous in overweight postmenopausal women. J Sport in Biomotor Sci, 8(15), 27-36. doi: https://doi.org/10.22080/JAEP.2017.8618.1405
Stewart, T.L., & Ralston, S.H. (2000). Role of genetic factors in the pathogenesis of osteoporosis. Journal of Endocrinology, 166, 235-245. PubMed doi: https://doi.org/10.1677/joe.0.1660235
Strath, S.J., Kaminsky, L.A., Ainsworth, B.E., Ekelund, U., Freedson, P.S., Gary, R.A., . . . Swartz, A.M. (2013). Guide to the assessment of physical activity: Clinical and research applications: a scientific statement from the American Heart Association. Circulation, 128, 2259-79. doi: https://doi.org/10.1161/01.cir.0000435708.67487
Tartibian, B., Afsargharehbagh, R., Malandish, A., Sheikhlou, Z (2018a). Assessment of electrocardiogram indices in postmenopausal women: effects of aerobic exercise and detraining. Int J Basic Sci Med, 3(1), 38-45. doi: https://doi.org/10.15171/ijbsm.2018.07
Tartibian, B., Botelho Teixeira, AM., & Baghaiee, B. (2015). Moderate intensity exercise is associated with decreased angiotensin-converting enzyme, increased β2-adrenergic receptor gene expression, and lower blood pressure in middle-aged men. Journal of Aging and Physical Activity, 23, 212-220. PubMed doi: https://doi.org/10.1123/japa.2013-0136
Tartibian, B., Hajizadeh Maleki, B., Kanaley, J., & Sadeghi, K. (2011) Long-term aerobic exercise and omega-3 supplementation modulate osteoporosis through inflammatory mechanisms in post-menopausal women: a randomized, repeated measures study. Nutrition & Metabolism (London), 15, 71. PubMed doi: https://doi.org/10.1186/1743-7075-8-71
Tartibian, B., Malandish, A., Afsargharehbagh, R., Eslami, R., Sheikhlou, Z (2018b). Assessment of Hepatic and Lipid Profiles Following 12 Weeks of Aerobic Exercise in Overweight Postmenopausal Women. Int J Basic Sci Med, 3(1), 38-45. doi: https://doi.org/10.15171/ijbsm.2018.28
VanDriel, M., & VanLeeuwen, J.P. (2014). Vitamin D endocrine system and osteoblasts. BoneKEy Reports, 3, 493. PubMed doi: https://doi.org/10.1038/bonekey.2013.227
Wang, B.L., Dai, C.L., Quan, J.X., Zhu, Z.F., Zheng, F., Zhang, H.X., . . . Qiu, M.C. (2006). Parathyroid hormone regulates osterix and Runx2 mRNA expression predominantly through protein kinase A signaling in osteoblast-like cells. Journal of Endocrinological Investigation, 29, 101-8. PubMed doi: https://doi.org/10.1007/BF03344081
Wang, Y., Zhu, J., & DeLuca, H.F. (2014). Identification of the vitamin D receptor in osteoblasts and chondrocytes but not osteoclasts in mouse bone. Journal of Bone and Mineral Research, 29, 685-92. PubMed doi: https://doi.org/10.1002/jbmr.2081
Woeckel, V.J., Bruedigam, C., Koedam, M., Chiba, H., van der Eerden, B.C., & van Leeuwen, J,P. (2013).1α,25-dihydroxyvitamin D3 and rosiglitazone synergistically enhance osteoblast-mediated mineralization. Gene, 512, 438-43. doi: https://doi.org/10.1016/j.gene.2012.07.051
Yavropoulou, M.P., Michopoulos, A., & Yovos, J.G. (2017). PTH and PTHR1 in osteocytes. New insights into old partners. Hormones (Athens), 16, 150-160. PubMed doi: https://doi.org/10.14310/horm.2002.1730
Yu, P.A., Hsu, W.H., Hsu, W.B., Kuo, L.T., Lin, Z.R., Shen, W.J., & Hsu, R.W. (2019). The effects of high impact exercise intervention on bone mineral density, physical fitness, and quality of life in postmenopausal women with osteopenia: A retrospective cohort study. Medicine (Baltimore), 98, e14898. PubMed doi: https://doi.org/10.1097/MD.0000000000014898
Zhou, S., Glowacki, J., Kim, S.W., Hahne, J., Geng, S., Mueller, S.M., . . . LeBoff, M.S. (2013). Clinical characteristics influence in vitro action of 1,25-Dihydroxyvitamin D3 in human marrow stromal cells. Journal of Bone and Mineral Research, 27, 1992–2000. PubMed doi: https://doi.org/10.1002/jbmr.1655
 
 
 
Journal of Exercise & Organ Cross Talk
Volume 1, Issue 2
September 2021
Pages 66-76

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History

  • Receive Date: 01 June 2021
  • Revise Date: 20 July 2021
  • Accept Date: 10 August 2021

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