Vesnu Publications (Farzanegan Radandish) Journal of Exercise & Organ Cross Talk 2783-2074 Articles in Press 2026 09 01 The effect of aerobic interval-style continuous training combined with CoQ10 supplementation on MDA and TGF-β levels in inactive obese men 140 146 245761 10.22122/jeoct.2026.577937.1197 EN Peyman Akraminia Department of exercise physiology, Faculty of Physical Education and Sports Sciences, Islamic Azad University, South Tehran Branch, Iran. 0000-0002-1908-1846 Abdolali Banaiefar Associated Professor, Department of exercise physiology, ST.C., Islamic Azad university, Tehran, Iran. Vahid Imanipour 3Department of Physical Education, Pa.C., Islamic Azad University, Parand, Iran. Hadi Khoran Department of Physical Education, ZARA.C., Islamic Azad University, Markazi, Iran. Journal Article 2026 02 24 Obesity is associated with chronic low-grade inflammation and elevated oxidative stress. This study investigated the independent and combined effects of an 8-week aerobic interval-style continuous training program and CoQ10 supplementation on serum TGF-β and MDA concentrations in inactive obese men. Sixty inactive obese men (aged 30–45 years) were randomly assigned to four groups (n=15 each): control (CONT), CoQ10 supplementation (SUP; 100 mg/day), training (TR), and combined training + supplementation (TR+SUP). The supervised training protocol was performed three sessions per week for 8 weeks with progressive intensity. Fasting venous blood samples were collected before and after the intervention. Serum MDA was measured using colorimetric assay, and TGF-β was quantified via ELISA. Data was analyzed using two-way repeated-measures ANOVA. Significant time × exercise interactions were observed for both MDA (F(1,56) = 28.793, p < 0.001) and TGF-β (F(1,56) = 30.617, p < 0.001), with reductions in the exercise groups (MDA: exercise groups from 210 nmol/L to 156 nmol/L; TGF-β: from 35 nmol/L to 30 pg/mL). Time × supplementation interactions were also significant but smaller (MDA: η_p² = 0.082; TGF-β: η_p² = 0.068). No significant three-way interaction was detected for either marker. These findings highlight aerobic interval-style continuous training as a cornerstone intervention for mitigating obesity-related oxidative stress and fibrotic signaling. CoQ10 supplementation produced a small independent reduction in both markers, but no synergistic or additive interaction with exercise was observed.