Vesnu Publications (Farzanegan Radandish) Journal of Exercise & Organ Cross Talk 2783-2074 6 2 2026 06 30 High-intensity interval training upregulates adiponectin receptor 1 expression and modulates serum antioxidant enzymes in a Murine model of breast cancer 73 81 242570 10.22122/jeoct.2026.578166.1198 EN Samira Neshati Department of Physical Education and Sport Sciences, SR.C., Islamic Azad university, Tehran, Iran. 0000-0002-5992-5564 Mandana Gholami Department of Physical Education and Sport Sciences, SR.C., Islamic Azad university, Tehran, Iran. 0000-0001-8960-4123 Hossein Shirvani Exercise Physiology Research Center, life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. 0000-0002-0696-958X Farshad Ghazalian Department of Physical Education and Sport Sciences, SR.C., Islamic Azad university, Tehran, Iran. 0000-0002-5805-0559 Hossein Abednatanzi Department of Physical Education and Sport Sciences, SR.C., Islamic Azad university, Tehran, Iran. 0000-0001-6638-1131 Journal Article 2026 02 25 This study aimed to investigate the effects of high-intensity interval training (HIIT) on Adiponectin receptor 1 (AdipR1) gene expression in breast tumor tissue and serum levels of glutathione peroxidase (GPX) and glutathione reductase (GR) in a murine model of breast cancer. Sixteen female BALB/c mice were inoculated subcutaneously with 4T1 murine mammary carcinoma cells (5 × 10⁵ cells/mouse). One week post-inoculation, mice were randomly assigned to either a tumor-bearing control group (Tumor, n=8) or a tumor-bearing group subjected to HIIT (Tumor+HIIT, n=8). The HIIT protocol was performed on a motor-driven treadmill five days/week for four weeks, consisting of six 2-minute high-intensity intervals (18–25 m/min, 80–90% VO₂max) interspersed with 3-minute active recovery periods (5–9 m/min). Twenty-four hours after the final session, tumor tissues were excised for AdipR1 gene expression analysis via quantitative real-time PCR (2^-ΔΔCT method), and serum samples were collected for assessment of GPX and GR levels using ELISA. Statistical comparisons were performed using independent samples t-tests (p<0.05). HIIT significantly upregulated AdipR1 gene expression in breast tumor tissue compared to the control group (p<0.0001). Serum GPX levels were significantly decreased in the Tumor+HIIT group compared to the Tumor control group (p<0.0001). However, no significant difference was observed in serum GR levels between the two groups (p=0.7499). These findings suggest that HIIT may influence breast cancer progression through adiponectin-mediated pathways and oxidative stress regulation, providing a potential non-pharmacological adjunctive strategy for breast cancer management. Further studies are warranted to elucidate the underlying molecular mechanisms and clinical implications. https://www.jeoct.com/article_242570_855d557d897b3bbd1ec7b7863c3ec61b.pdf