Journal of Exercise & Organ Cross Talk

Abstract Obesity is associated with chronic low-grade inflammation and elevated oxidative stress. This study investigated the independent and combined effects of an 8-week aerobic interval-style continuous training program and CoQ10 supplementation on serum TGF-β and MDA concentrations in inactive obese men. Sixty inactive obese men (aged 30–45 years) were randomly assigned to four groups (n=15 each): control (CONT), CoQ10 supplementation (SUP; 100 mg/day), training (TR), and combined training + supplementation (TR+SUP). The supervised training protocol was performed three sessions per week for 8 weeks with progressive intensity. Fasting venous blood samples were collected before and after the intervention. Serum MDA was measured using colorimetric assay, and TGF-β was quantified via ELISA. Data was analyzed using two-way repeated-measures ANOVA. Significant time × exercise interactions were observed for both MDA (F(1,56) = 28.793, p < 0.001) and TGF-β (F(1,56) = 30.617, p < 0.001), with reductions in the exercise groups (MDA: exercise groups from 210 nmol/L to 156 nmol/L; TGF-β: from 35 nmol/L to 30 pg/mL). Time × supplementation interactions were also significant but smaller (MDA: η_p² = 0.082; TGF-β: η_p² = 0.068). No significant three-way interaction was detected for either marker. These findings highlight aerobic interval-style continuous training as a cornerstone intervention for mitigating obesity-related oxidative stress and fibrotic signaling. CoQ10 supplementation produced a small independent reduction in both markers, but no synergistic or additive interaction with exercise was observed.

Abstract It has been proven that strenuous endurance training increases oxidative stress in body. This study investigated the effects of curcumin supplementation and light resistance training during 8 weeks of endurance training on muscle and serum antioxidant capacity and lipid peroxidation of male wistar rats. 44 male Wistar rats (weight: 254.31±17.72 g and age: 8 weeks) were randomly divided to 6 groups; Control (n=6, sham), Curcumin (n=6), Endurance (n=8), Endurance-curcumin (n=8), Endurance-Resistance (n=8), and Endurance-curcumin-resistance (n=8). Endurance training performed on rodent treadmill for 8 weeks and 5 sessions a week. The speed and duration of running were 10 m/min and 30 min at first week. The intensity and duration reached to the 35 m/min and 70 min up to the last week. Resistance training (8 weeks, 2 sessions / week) performed on vertical ladder (with 30-70% BW). The animals received curcumin supplement by sub peritoneal injection (8 weeks, 3 sessions / week, 30 mg/kg.Bw). Superoxide dismutase (SOD) enzyme activity was measured by Elisa kit and Malondialdehyde (MDA) was measured by the thiobarbituric acid reactive substances (TBARS).  The results of this study showed that strenuous endurance training (p<0.05) reduces the serum levels of SOD significantly, and caused a significant increase in the lipid peroxidation (MDA in muscle and serum). Curcumin supplementation and light resistance training could increase antioxidant enzymes activity (SOD) and decrease the MDA levels. The prolonged strenuous endurance training can induce oxidative stress and curcumin supplementation along with light resistance training could restore antioxidant enzymes activity and decrease the MDA levels.