The effect of exercise training with Nano selenium supplementation on LDHA, LDHB genes and LDHA/LDHB ratio at breast tumor tissue of mouse model
Volume 6, Issue 2, Spring 2026, Pages 99-106
https://doi.org/10.22122/jeoct.2026.579301.1200
Mohadeseh Akaberi, Mandana Gholami, Hossein Shirvani, Farshad Ghazalian, Hossein Abednatanzi
Abstract This study investigated the effects of high intensity interval training (HIIT), Nano-selenium supplementation, and their combination on the expression of LDHA, LDHB, and the LDHA/LDHB ratio in mice breast tumor tissue. Female mice (n=32) were inoculated with mammary adenocarcinoma cells (4T1) and randomly assigned to four groups (n=8 each): tumor control (Tu), tumor+HIIT (Tu+Ex), tumor + Nano-selenium (Tu+Nsel, 2 mg/kg/day orally), and tumor+HIIT+Nano-selenium (Tu+Ex+Nsel). HIIT was performed on a treadmill (30 min/day, 5 days/week) for four weeks. One-way ANOVA revealed significant differences among groups for LDHA expression (F=38.66, p<0.0001). Compared to the Tu group, all intervention groups (Tu+Ex,Tu+Nsel, and Tu+Ex+Nsel) showed a significant increase in LDHA expression (p<0.05). The greatest increase was observed in the combined treatment group (Tu+Ex+Nsel), which was significantly higher than both Tu+Ex and Tu+Nsel (p<0.001). For the LDHA/LDHB ratio, a significant overall effect was found (F=163.87, p<0.0001). The Tu+Nsel group exhibited a significant increase in the ratio compared to the Tu group (p<0.05), whereas both Tu+Ex and Tu+Ex+Nsel showed a significant decrease in the ratio (p<0.05). The ratio in the Tu+Nsel group was also significantly higher than in the two exercise containing groups (p<0.05). HIIT and Nano-selenium independently upregulate LDHA expression in breast tumor tissue, with an additive effect when combined. However, only Nano-selenium alone increased the LDHA/LDHB ratio, while exercise-based interventions (with or without Nano-selenium) decreased this ratio. These findings suggest that exercise and Nano-selenium differentially shift the balance between LDHA and LDHB, potentially influencing tumor lactate metabolism and the tumor microenvironment.