Journal of Exercise & Organ Cross Talk

Abstract Hepatic gene expression of inflammatory and growth factors such as IL-8 and bFGF2 may be modulated in melanoma metastasis. Non-pharmacological interventions like exercise and anti-inflammatory supplements represent potential complementary strategies for modification. This study aimed to investigate the effects of aerobic exercise, pineapple extract supplementation, and their combination on the hepatic expression of CXCL2/IL-8 HOMOLOG and bFGF2 genes in a murine melanoma model. Melanoma-bearing mice were allocated into four groups (n=5 per group): Control, Aerobic Exercise, Pineapple Extract Supplement, and Aerobic Exercise+Pineapple Extract. After the intervention period, liver tissue was analyzed for CXCL2/IL-8 HOMOLOG and bFGF2 gene expression via one-way ANOVA and Tukey HSD test. Pearson correlation assessed the relationship between the two genes. A significant difference was observed in CXCL2/IL-8 HOMOLOG gene expression between groups (F=4.211, p=0.0239). Post hoc analysis revealed that only the combined Aerobic Exercise + Pineapple Extract group showed a significant decrease in hepatic CXCL2/IL-8 HOMOLOG compared to the Cancer Control group (p=0.0251). In contrast, no significant difference was found in bFGF2 gene expression across groups (F=1.425, p=0.2745). Correlation analysis indicated a significant negative relationship between CXCL2/IL-8 HOMOLOG and bFGF2 exclusively in the Cancer Control group (r=-0.948, p=0.013). The combination of aerobic exercise and pineapple extract supplementation significantly reduces hepatic CXCL2/IL-8 HOMOLOG expression in melanoma-bearing mice, suggesting a potential synergistic effect in modulating the hepatic inflammatory microenvironment. The distinct lack of effect on bFGF2 and the specific negative correlation in controls highlight pathway-selective responses.

Abstract This study investigated the effects of resistance training and pineapple extract consumption on intratumoral NF-κB and LIN28B gene expression and serum TNF-α levels in a murine C57 melanoma model. Twenty C57BL/6 mice were allocated into four groups (n=5/group): melanoma tumor control (MT), MT with resistance training (MT+RT), MT with pineapple extract (MT+PJ), and MT with combined intervention (MT+RT+PJ). The RT protocol and PJ administration (300 mg/kg/day via gavage) were conducted for six weeks’ post-tumor induction. Serum TNF-α was quantified by ELISA, and tumor gene expression of NF-κB and LIN28B was analyzed via RT-PCR. Data were analyzed using one-way ANOVA followed by Tukey's post hoc test. All three intervention groups exhibited a significant downregulation of NF-κB and LIN28B gene expression in tumor tissue compared to the MT control group (p<0.05). Conversely, serum TNF-α levels were significantly elevated in the intervention groups relative to the control (p<0.05). Resistance training and pineapple extract consumption, both individually and in combination, significantly modulated pro-tumorigenic pathways by suppressing intratumoral NF-κB and LIN28B expression, despite an observed increase in systemic TNF-α.

Abstract Exercise and the use of anti-inflammatory supplements are effective in controlling inflammation and tissue damage in diabetes. Therefore, the aim of the present study was to investigate the effect of 8 weeks of interval training and royal jelly on the expression of renal inflammatory genes and insulin resistance in diabetic rats. Thirty-two male Wistar rats were used in this study. Diabetes was induced by a high-fat diet for 20 weeks followed by intraperitoneal injection of freshly prepared STZ solution in saline (25 mg/kg). Then the rats were randomly divided into 4 groups: Diabetes (DI), DI+ HIIT, DI+ royal jelly (RJ), and DI+ HIIT+ RJ. Royal jelly groups were given royal jelly at a dose of 100 mg/kg diluted in distilled water and gavage 5 days a week. The exercise program consisted of eight weeks of HIIT training, five sessions per week with a gradual increase in intensity intervals from 22 to 38 meters per minute (80 to 90% of Vo2max) and rest intervals at a speed of 16 to 22 meters per minute (50 to 56% of Vo2max). At the end of the research, rats were euthanized and kidney tissue was removed to measure the expression of TNF-α and NF-κB genes. The DI+HIIT (p=0.0006), DI+RJ (p=0.0011) and DI+HIIT+RJ (p<0.0001) groups showed a significant decrease in HOMA-IR compared to the diabetes control group. The DI+HIIT, DI+RJ and DI+HIIT+RJ groups showed a significant decrease in TNF-α and NF-κB gene expression in kidney tissue compared to the diabetes control group. In conclusion, 8 weeks of HIIT and/or royal jelly supplementation significantly ameliorated insulin resistance and suppressed renal pro-inflammatory gene expression (TNF-α, NF-κB) in diabetic rats, with the combined approach showing the greatest efficacy.