Journal of Exercise & Organ Cross Talk

Abstract Glioblastoma multiforme (GBM) exhibits dysregulated p53 tumor-suppressor signaling, driving therapeutic resistance; exercise and nutraceuticals represent promising adjuvants for pathway modulation. This study investigated resistance training and nanocurcumin in an orthotopic GBM rat model (n=40), with groups including healthy controls, cancer controls, nanocurcumin (80 mg/kg/day), resistance training (ladder climbing, 50% BW + 30g, 3×/week), and combined intervention. After 4 weeks, tumor tissue analysis revealed resistance training significantly reduced p53 mRNA expression versus cancer controls (1.8 ± 0.2 vs. 3.1 ± 0.3; p = 0.021), while all interventions suppressed p21 (combination group: 1.2 ± 0.1 vs. control 4.0 ± 0.4; p < 0.001), demonstrating 70% greater p21 inhibition in the combined group versus monotherapies (p < 0.01). The synergistic p21 downregulation indicates potent disruption of the G1/S cell-cycle checkpoint, likely mediated through inter-organ cross-talk along the muscle-liver-brain axis—where exercise-induced myokines (IL-6, BDNF) mitigate tumor oxidative stress, nanocurcumin suppresses hepatic inflammatory mediators, and hemodynamic adaptations enhance blood-brain barrier penetration. These findings position resistance training and nanocurcumin as a novel non-pharmacological adjuvant strategy to potentiate conventional glioma therapies by leveraging systemic physiological communication.

Abstract Impaired cell internal settings and excessive proliferation causes the occurrence of diverse ranges of syndrom and diseases. The pathological stress underlying these conditions triggers persistent flux through multiple intracellular signaling pathways amongst MAPK/ERK as master regulator. Regarding the anti-inflammatory effects of muscle contraction induced myokines and nano-curcumin supplementation, we aimed to investigate the effects of aerobic training and nano-curcumin supplementation on RAS and ERK gene expression in rat muscle tissue. In this experimental study, 32 male wistar rats (aged 4-6 weeks, 130-150 g) were randomly assigned into 4 groups, including Control (C), Moderate Intensity Continious Training (MICT), Nano-Curcumin Supplementation (NCS) and Moderate Intensity Continious Training + Nano-curcumin (MICT+NCS). The training groups implemented the MICT protocol consisted of running at a velocity of 18-20 m/min, 5 days a week and for a total time of 4 weeks. The Supplement groups received 80 mg/kg/day through oral gavage. Regarding the results of one-way ANOVA, 4 weeks of moderate intensity aerobic exercise and Nano-curcumin supplementation led to a significant difference in the RAS (P=0.001) and ERK (P=0.01) gene expression levels in muscle tissue of rats among the study groups. Also, the results of the Bonferronie test showed that implementation of 4 weeks of MICT along with nano-curcumin supplementation alleviated the RAS/ERK gene expression levels, meanwhile nano-curcumin more efficiently down-regulated the pathway; suggesting that nano-curcumin can be an effective ergogenic aid for improving anti-inflmmatory properties through RAS/ERK signaling pathway.

Abstract Steatosis is the most important cause for chronic liver disease, from simple steatosis to advanced stages such as liver fibrosis and cirrhosis and liver cancer. In this study, the effect of MICT and probiotic consumption on IL15 gene expression in animal model of non-alcoholic steatosis was examined. This study is an experimental laboratory study. Thirty-two male wistar rats were divided into 4 groups of 8 in the healthy (normal diet), steatosis, steatosis + probiotic, steatosis + probiotic +MICT groups and tested for 8 weeks. The exercise protocol was as follows: in the first week from 10 minutes of running at a speed of 18 meters per minute to the eighth week with 60 minutes of running at a speed of 28 meters per minute. Consumption of probiotics; Relevant groups received 109 CFU / ml of Lactobacillus rhamnosus GG by gavage daily for 5 weeks and 5 days a week. Statistical calculation of this study was performed using SPSS 25 software. Probiotic consumption and moderate-intensity continuous training significantly increased IL-15 (p =0.000) and significantly decreased TG (p =0.000) in the non-alcoholic steatosis animal model. It seems moderate-intensity continuous training with probiotic consumption can improve the liver function of non-alcoholic fatty liver patients.